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Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis

Context: The roots of Lophira lanceolata Van Tiegh. Ex Keay (Ochnaceae) have numerous medicinal values in the Central African region. Even though the MeOH extract of the roots has shown antimycobacterial activities, the constituents responsible for this inhibitory activity remain unknown. Objective:...

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Autores principales: Nkot, Jeanne Louise, Ngono Bikobo, Dominique Serge, Abouem A Zintchem, Auguste, Nyemeck, Norbert Mbabi, Moni Ndedi, Esther Del Florence, Betote Diboué, Patrick Hervé, Pegnyemb, Dieudonné Emmanuel, Bochet, Christian G., Koert, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130701/
https://www.ncbi.nlm.nih.gov/pubmed/29969355
http://dx.doi.org/10.1080/13880209.2018.1476559
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author Nkot, Jeanne Louise
Ngono Bikobo, Dominique Serge
Abouem A Zintchem, Auguste
Nyemeck, Norbert Mbabi
Moni Ndedi, Esther Del Florence
Betote Diboué, Patrick Hervé
Pegnyemb, Dieudonné Emmanuel
Bochet, Christian G.
Koert, Ulrich
author_facet Nkot, Jeanne Louise
Ngono Bikobo, Dominique Serge
Abouem A Zintchem, Auguste
Nyemeck, Norbert Mbabi
Moni Ndedi, Esther Del Florence
Betote Diboué, Patrick Hervé
Pegnyemb, Dieudonné Emmanuel
Bochet, Christian G.
Koert, Ulrich
author_sort Nkot, Jeanne Louise
collection PubMed
description Context: The roots of Lophira lanceolata Van Tiegh. Ex Keay (Ochnaceae) have numerous medicinal values in the Central African region. Even though the MeOH extract of the roots has shown antimycobacterial activities, the constituents responsible for this inhibitory activity remain unknown. Objective: Phytochemical investigation of the MeOH root extract of L. lanceolata and determination of the antimycobacterial activities of that extract and constituents against the growth of Mycobacterium tuberculosis. Materials and methods: Column chromatography was used to provide bioactive phytoconstituents. Those compounds were elucidated using MS and NMR spectroscopic data. Antimycobacterial screening of the extract (4.882–5000 µg/mL in DMSO during 24 h at 37 °C) and isolated compounds (0.244–250 µg/mL in DMSO during 24 h at 37 °C) was performed by microplate alamar blue assay (MABA) against two mycobacterial strains. Results: The investigation of L. lanceolata MeOH roots extract provided of mixture of unseparated biflavonoids with a newly described one, dihydrolophirone A (1a) associated to lophirone A (1b). The bioactive compounds that effectively inhibited the growth of M. tuberculosis AC45 were found to be compounds 1 and 2. They exhibited MIC values of 31.25 and 15.75 µg/mL, respectively, and their MIC was found to be 62.5 µg/mL against resistant strain AC83. Discussion and conclusions: It is clearly evident from the results obtained that the mycobacterial activity of L. lanceolata could be related mainly to its steroid and flavonoid contents. Therefore, this study suggests the potential of the above-mentioned classes of compounds as promising candidate agents for developing new anti-tuberculosis drugs.
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spelling pubmed-61307012018-09-27 Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis Nkot, Jeanne Louise Ngono Bikobo, Dominique Serge Abouem A Zintchem, Auguste Nyemeck, Norbert Mbabi Moni Ndedi, Esther Del Florence Betote Diboué, Patrick Hervé Pegnyemb, Dieudonné Emmanuel Bochet, Christian G. Koert, Ulrich Pharm Biol Research Article Context: The roots of Lophira lanceolata Van Tiegh. Ex Keay (Ochnaceae) have numerous medicinal values in the Central African region. Even though the MeOH extract of the roots has shown antimycobacterial activities, the constituents responsible for this inhibitory activity remain unknown. Objective: Phytochemical investigation of the MeOH root extract of L. lanceolata and determination of the antimycobacterial activities of that extract and constituents against the growth of Mycobacterium tuberculosis. Materials and methods: Column chromatography was used to provide bioactive phytoconstituents. Those compounds were elucidated using MS and NMR spectroscopic data. Antimycobacterial screening of the extract (4.882–5000 µg/mL in DMSO during 24 h at 37 °C) and isolated compounds (0.244–250 µg/mL in DMSO during 24 h at 37 °C) was performed by microplate alamar blue assay (MABA) against two mycobacterial strains. Results: The investigation of L. lanceolata MeOH roots extract provided of mixture of unseparated biflavonoids with a newly described one, dihydrolophirone A (1a) associated to lophirone A (1b). The bioactive compounds that effectively inhibited the growth of M. tuberculosis AC45 were found to be compounds 1 and 2. They exhibited MIC values of 31.25 and 15.75 µg/mL, respectively, and their MIC was found to be 62.5 µg/mL against resistant strain AC83. Discussion and conclusions: It is clearly evident from the results obtained that the mycobacterial activity of L. lanceolata could be related mainly to its steroid and flavonoid contents. Therefore, this study suggests the potential of the above-mentioned classes of compounds as promising candidate agents for developing new anti-tuberculosis drugs. Taylor & Francis 2018-07-03 /pmc/articles/PMC6130701/ /pubmed/29969355 http://dx.doi.org/10.1080/13880209.2018.1476559 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nkot, Jeanne Louise
Ngono Bikobo, Dominique Serge
Abouem A Zintchem, Auguste
Nyemeck, Norbert Mbabi
Moni Ndedi, Esther Del Florence
Betote Diboué, Patrick Hervé
Pegnyemb, Dieudonné Emmanuel
Bochet, Christian G.
Koert, Ulrich
Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis
title Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis
title_full Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis
title_fullStr Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis
title_full_unstemmed Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis
title_short Antitubercular evaluation of root extract and isolated phytochemicals from Lophira lanceolata against two resistant strains of Mycobacterium tuberculosis
title_sort antitubercular evaluation of root extract and isolated phytochemicals from lophira lanceolata against two resistant strains of mycobacterium tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130701/
https://www.ncbi.nlm.nih.gov/pubmed/29969355
http://dx.doi.org/10.1080/13880209.2018.1476559
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