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Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy

Context: Royal jelly (RJ) has long been used to promote human health. Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins. Materials and methods: Balb/c mice treated orally...

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Autores principales: Guendouz, Malika, Haddi, Abir, Grar, Hadria, Kheroua, Omar, Saidi, Djamel, Kaddouri, Hanane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130715/
https://www.ncbi.nlm.nih.gov/pubmed/28982287
http://dx.doi.org/10.1080/13880209.2017.1383487
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author Guendouz, Malika
Haddi, Abir
Grar, Hadria
Kheroua, Omar
Saidi, Djamel
Kaddouri, Hanane
author_facet Guendouz, Malika
Haddi, Abir
Grar, Hadria
Kheroua, Omar
Saidi, Djamel
Kaddouri, Hanane
author_sort Guendouz, Malika
collection PubMed
description Context: Royal jelly (RJ) has long been used to promote human health. Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins. Materials and methods: Balb/c mice treated orally for seven days with RJ at doses of 0.5, 1 and 1.5 g/kg were sensitized intraperitoneally with β-lactoglobulin (β-Lg). Serum IgG and IgE anti-β-Lg were determined by an enzyme-linked immunosorbent assay (ELISA). Plasma histamine levels, symptom scores and body temperature were determined after in vivo challenge to β-Lg. Jejunums were used for assessment of local anaphylactic responses by an ex vivo study in Ussing chambers and morphologic changes by histological analysis. Results: RJ significantly decreased serum IgG (31.15–43.78%) and IgE (64.28–66.6%) anti-β-Lg and effectively reduced plasma histamine level (66.62–67.36%) (p < 0.001) at all the doses tested. Additionally, no clinical symptoms or body temperature drops were observed in RJ-pretreated mice. Interestingly, RJ significantly reduced (p < 0.001) intestinal dysfunction by abolishing the secretory response (70.73–72.23%) induced by sensitization and prevented length aberrations of jejunal villi by 44.32–59.01% (p < 0.001). Discussion and conclusions: We speculate that using RJ may help prevent systemic and anaphylactic response in allergic mice. These effects may be related to its inhibitory effects on the degranulation of mast cells.
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spelling pubmed-61307152018-09-27 Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy Guendouz, Malika Haddi, Abir Grar, Hadria Kheroua, Omar Saidi, Djamel Kaddouri, Hanane Pharm Biol Research Article Context: Royal jelly (RJ) has long been used to promote human health. Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins. Materials and methods: Balb/c mice treated orally for seven days with RJ at doses of 0.5, 1 and 1.5 g/kg were sensitized intraperitoneally with β-lactoglobulin (β-Lg). Serum IgG and IgE anti-β-Lg were determined by an enzyme-linked immunosorbent assay (ELISA). Plasma histamine levels, symptom scores and body temperature were determined after in vivo challenge to β-Lg. Jejunums were used for assessment of local anaphylactic responses by an ex vivo study in Ussing chambers and morphologic changes by histological analysis. Results: RJ significantly decreased serum IgG (31.15–43.78%) and IgE (64.28–66.6%) anti-β-Lg and effectively reduced plasma histamine level (66.62–67.36%) (p < 0.001) at all the doses tested. Additionally, no clinical symptoms or body temperature drops were observed in RJ-pretreated mice. Interestingly, RJ significantly reduced (p < 0.001) intestinal dysfunction by abolishing the secretory response (70.73–72.23%) induced by sensitization and prevented length aberrations of jejunal villi by 44.32–59.01% (p < 0.001). Discussion and conclusions: We speculate that using RJ may help prevent systemic and anaphylactic response in allergic mice. These effects may be related to its inhibitory effects on the degranulation of mast cells. Taylor & Francis 2017-10-05 /pmc/articles/PMC6130715/ /pubmed/28982287 http://dx.doi.org/10.1080/13880209.2017.1383487 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Guendouz, Malika
Haddi, Abir
Grar, Hadria
Kheroua, Omar
Saidi, Djamel
Kaddouri, Hanane
Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
title Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
title_full Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
title_fullStr Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
title_full_unstemmed Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
title_short Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
title_sort preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130715/
https://www.ncbi.nlm.nih.gov/pubmed/28982287
http://dx.doi.org/10.1080/13880209.2017.1383487
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