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Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy
Context: Royal jelly (RJ) has long been used to promote human health. Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins. Materials and methods: Balb/c mice treated orally...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130715/ https://www.ncbi.nlm.nih.gov/pubmed/28982287 http://dx.doi.org/10.1080/13880209.2017.1383487 |
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author | Guendouz, Malika Haddi, Abir Grar, Hadria Kheroua, Omar Saidi, Djamel Kaddouri, Hanane |
author_facet | Guendouz, Malika Haddi, Abir Grar, Hadria Kheroua, Omar Saidi, Djamel Kaddouri, Hanane |
author_sort | Guendouz, Malika |
collection | PubMed |
description | Context: Royal jelly (RJ) has long been used to promote human health. Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins. Materials and methods: Balb/c mice treated orally for seven days with RJ at doses of 0.5, 1 and 1.5 g/kg were sensitized intraperitoneally with β-lactoglobulin (β-Lg). Serum IgG and IgE anti-β-Lg were determined by an enzyme-linked immunosorbent assay (ELISA). Plasma histamine levels, symptom scores and body temperature were determined after in vivo challenge to β-Lg. Jejunums were used for assessment of local anaphylactic responses by an ex vivo study in Ussing chambers and morphologic changes by histological analysis. Results: RJ significantly decreased serum IgG (31.15–43.78%) and IgE (64.28–66.6%) anti-β-Lg and effectively reduced plasma histamine level (66.62–67.36%) (p < 0.001) at all the doses tested. Additionally, no clinical symptoms or body temperature drops were observed in RJ-pretreated mice. Interestingly, RJ significantly reduced (p < 0.001) intestinal dysfunction by abolishing the secretory response (70.73–72.23%) induced by sensitization and prevented length aberrations of jejunal villi by 44.32–59.01% (p < 0.001). Discussion and conclusions: We speculate that using RJ may help prevent systemic and anaphylactic response in allergic mice. These effects may be related to its inhibitory effects on the degranulation of mast cells. |
format | Online Article Text |
id | pubmed-6130715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-61307152018-09-27 Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy Guendouz, Malika Haddi, Abir Grar, Hadria Kheroua, Omar Saidi, Djamel Kaddouri, Hanane Pharm Biol Research Article Context: Royal jelly (RJ) has long been used to promote human health. Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins. Materials and methods: Balb/c mice treated orally for seven days with RJ at doses of 0.5, 1 and 1.5 g/kg were sensitized intraperitoneally with β-lactoglobulin (β-Lg). Serum IgG and IgE anti-β-Lg were determined by an enzyme-linked immunosorbent assay (ELISA). Plasma histamine levels, symptom scores and body temperature were determined after in vivo challenge to β-Lg. Jejunums were used for assessment of local anaphylactic responses by an ex vivo study in Ussing chambers and morphologic changes by histological analysis. Results: RJ significantly decreased serum IgG (31.15–43.78%) and IgE (64.28–66.6%) anti-β-Lg and effectively reduced plasma histamine level (66.62–67.36%) (p < 0.001) at all the doses tested. Additionally, no clinical symptoms or body temperature drops were observed in RJ-pretreated mice. Interestingly, RJ significantly reduced (p < 0.001) intestinal dysfunction by abolishing the secretory response (70.73–72.23%) induced by sensitization and prevented length aberrations of jejunal villi by 44.32–59.01% (p < 0.001). Discussion and conclusions: We speculate that using RJ may help prevent systemic and anaphylactic response in allergic mice. These effects may be related to its inhibitory effects on the degranulation of mast cells. Taylor & Francis 2017-10-05 /pmc/articles/PMC6130715/ /pubmed/28982287 http://dx.doi.org/10.1080/13880209.2017.1383487 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Guendouz, Malika Haddi, Abir Grar, Hadria Kheroua, Omar Saidi, Djamel Kaddouri, Hanane Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
title | Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
title_full | Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
title_fullStr | Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
title_full_unstemmed | Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
title_short | Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
title_sort | preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130715/ https://www.ncbi.nlm.nih.gov/pubmed/28982287 http://dx.doi.org/10.1080/13880209.2017.1383487 |
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