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Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats

Context: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic ra...

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Autores principales: Xia, Ling-Ling, Zhu, Qi-Jin, Wu, Yong-Gui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130753/
https://www.ncbi.nlm.nih.gov/pubmed/29078720
http://dx.doi.org/10.1080/13880209.2017.1390589
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author Xia, Ling-Ling
Zhu, Qi-Jin
Wu, Yong-Gui
author_facet Xia, Ling-Ling
Zhu, Qi-Jin
Wu, Yong-Gui
author_sort Xia, Ling-Ling
collection PubMed
description Context: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic rats. Objectives: Current study investigates prevention of liver injury by TGP in diabetic rats and its mechanism was related to the inhibition of endoplasmic reticulum stress (ERS). Materials and methods: Fifty adult male rats were randomly divided into: Normal group, diabetic group, TGP (50, 100 and 200 mg/kg/day) treatment groups (n = 10 per group). At the end of the 8th week, the liver was removed for biochemical and histological examinations. Results: Compared with the diabetic group, administration of TGP at doses of 50, 100 and 200 mg/kg significantly prevented the increase of hepatic fibrosis score (ED(50) 139.4 mg/kg). Compared with diabetic group, TGP at doses of 50, 100 and 200 mg/kg showed an inhibition on the increased macrophage infiltration. MCP-1 and TNF-α mRNA and protein expression were significantly increased in diabetic group compared with normal group; TGP administration caused significant reduction of high levels of MCP-1 and TNF-α mRNA as well as protein levels. Also, TGP at all doses showed an inhibition on the increased GRP78 levels, p-Perk levels and p-Eif2α levels in liver from diabetic group. Discussion and conclusions: Our results indicate that TGP has potential as a treatment for diabetic liver injury attenuating liver lipid accumulation and inflammation as well as ERS induced by diabetic condition.
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spelling pubmed-61307532018-09-27 Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats Xia, Ling-Ling Zhu, Qi-Jin Wu, Yong-Gui Pharm Biol Research Article Context: Total glucosides of peony (TGP), compounds extracted from the dried roots of Paeonia lactiflora Pall, have been reported to have anti-inflammatory and antioxidative activities. However, the protective effect of TGP on liver injury and the underlying mechanisms remains unknown in diabetic rats. Objectives: Current study investigates prevention of liver injury by TGP in diabetic rats and its mechanism was related to the inhibition of endoplasmic reticulum stress (ERS). Materials and methods: Fifty adult male rats were randomly divided into: Normal group, diabetic group, TGP (50, 100 and 200 mg/kg/day) treatment groups (n = 10 per group). At the end of the 8th week, the liver was removed for biochemical and histological examinations. Results: Compared with the diabetic group, administration of TGP at doses of 50, 100 and 200 mg/kg significantly prevented the increase of hepatic fibrosis score (ED(50) 139.4 mg/kg). Compared with diabetic group, TGP at doses of 50, 100 and 200 mg/kg showed an inhibition on the increased macrophage infiltration. MCP-1 and TNF-α mRNA and protein expression were significantly increased in diabetic group compared with normal group; TGP administration caused significant reduction of high levels of MCP-1 and TNF-α mRNA as well as protein levels. Also, TGP at all doses showed an inhibition on the increased GRP78 levels, p-Perk levels and p-Eif2α levels in liver from diabetic group. Discussion and conclusions: Our results indicate that TGP has potential as a treatment for diabetic liver injury attenuating liver lipid accumulation and inflammation as well as ERS induced by diabetic condition. Taylor & Francis 2017-10-27 /pmc/articles/PMC6130753/ /pubmed/29078720 http://dx.doi.org/10.1080/13880209.2017.1390589 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xia, Ling-Ling
Zhu, Qi-Jin
Wu, Yong-Gui
Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
title Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
title_full Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
title_fullStr Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
title_full_unstemmed Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
title_short Hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
title_sort hepatoprotective effect of peony total glucosides and the underlying mechanisms in diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130753/
https://www.ncbi.nlm.nih.gov/pubmed/29078720
http://dx.doi.org/10.1080/13880209.2017.1390589
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