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Enhancer mapping uncovers phenotypic heterogeneity and evolution in patients with luminal breast cancer

The degree of intrinsic and interpatient phenotypic heterogeneity and its role in tumour evolution is poorly understood. Phenotypic drifts can be transmitted via inheritable transcriptional programs. Cell-type specific transcription is maintained through the activation of epigenetically-defined regu...

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Detalles Bibliográficos
Autores principales: Patten, Darren K., Corleone, Giacomo, Győrffy, Balázs, Perone, Ylenia, Slaven, Neil, Barozzi, Iros, Erdős, Edina, Saiakhova, Alina, Goddard, Kate, Vingiani, Andrea, Shousha, Sami, Pongor, Lőrinc Sándor, Hadjiminas, Dimitri J., Schiavon, Gaia, Barry, Peter, Palmieri, Carlo, Coombes, Raul C., Scacheri, Peter, Pruneri, Giancarlo, Magnani, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130800/
https://www.ncbi.nlm.nih.gov/pubmed/30038216
http://dx.doi.org/10.1038/s41591-018-0091-x
Descripción
Sumario:The degree of intrinsic and interpatient phenotypic heterogeneity and its role in tumour evolution is poorly understood. Phenotypic drifts can be transmitted via inheritable transcriptional programs. Cell-type specific transcription is maintained through the activation of epigenetically-defined regulatory regions including promoters and enhancers. Here we annotated the epigenome of 47 primary and metastatic oestrogen-receptor (ERα)-positive breast cancer clinical specimens and inferred phenotypic heterogeneity from the regulatory landscape, identifying key regulatory elements commonly shared across patients. Shared regions contain a unique set of regulatory information including the motif for the transcription factor YY1. We identify YY1 as a critical determinant of ERα transcriptional activity promoting tumour growth in most luminal patients. YY1 also contributes to the expression of genes mediating resistance to endocrine treatment. Finally, we used H3K27ac levels at active enhancer elements as a surrogate of intra-tumour phenotypic heterogeneity to track the expansion and contraction of phenotypic subpopulations throughout breast cancer progression. By tracking the clonality of SLC9A3R1-positive cells, a bona fide YY1-ERα-regulated gene, we show that endocrine therapies select for phenotypic clones underrepresented at diagnosis. Collectively, our data show that epigenetic mechanisms significantly contribute to phenotypic heterogeneity and evolution in systemically treated breast cancer patients.