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Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions

Decoding the information in mRNA during protein synthesis relies on tRNA adaptors, the abundance of which can affect the decoding rate and translation efficiency. To determine whether cells alter tRNA abundance to selectively regulate protein expression, we quantified changes in the abundance of ind...

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Autores principales: Torrent, Marc, Chalancon, Guilhem, de Groot, Natalia S., Wuster, Arthur, Babu, M. Madan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130803/
https://www.ncbi.nlm.nih.gov/pubmed/30181241
http://dx.doi.org/10.1126/scisignal.aat6409
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author Torrent, Marc
Chalancon, Guilhem
de Groot, Natalia S.
Wuster, Arthur
Babu, M. Madan
author_facet Torrent, Marc
Chalancon, Guilhem
de Groot, Natalia S.
Wuster, Arthur
Babu, M. Madan
author_sort Torrent, Marc
collection PubMed
description Decoding the information in mRNA during protein synthesis relies on tRNA adaptors, the abundance of which can affect the decoding rate and translation efficiency. To determine whether cells alter tRNA abundance to selectively regulate protein expression, we quantified changes in the abundance of individual tRNAs at different time points in response to diverse stress conditions in Saccharomyces cerevisiae. We found that the tRNA pool was dynamic and rearranged in a manner that facilitated selective translation of stress-related transcripts. Through genomic analysis of multiple data sets, stochastic simulations, and experiments with designed sequences of proteins with identical amino acids but altered codon usage, we showed that changes in tRNA abundance affected protein expression independently of factors such as mRNA abundance. We suggest that cells alter their tRNA abundance to selectively affect the translation rates of specific transcripts to increase the amounts of required proteins under diverse stress conditions.
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spelling pubmed-61308032019-03-04 Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions Torrent, Marc Chalancon, Guilhem de Groot, Natalia S. Wuster, Arthur Babu, M. Madan Sci Signal Stress Responses Decoding the information in mRNA during protein synthesis relies on tRNA adaptors, the abundance of which can affect the decoding rate and translation efficiency. To determine whether cells alter tRNA abundance to selectively regulate protein expression, we quantified changes in the abundance of individual tRNAs at different time points in response to diverse stress conditions in Saccharomyces cerevisiae. We found that the tRNA pool was dynamic and rearranged in a manner that facilitated selective translation of stress-related transcripts. Through genomic analysis of multiple data sets, stochastic simulations, and experiments with designed sequences of proteins with identical amino acids but altered codon usage, we showed that changes in tRNA abundance affected protein expression independently of factors such as mRNA abundance. We suggest that cells alter their tRNA abundance to selectively affect the translation rates of specific transcripts to increase the amounts of required proteins under diverse stress conditions. American Association for the Advancement of Science 2018-09-04 2018 /pmc/articles/PMC6130803/ /pubmed/30181241 http://dx.doi.org/10.1126/scisignal.aat6409 Text en © 2018 The Authors, some rights reserved https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Stress Responses
Torrent, Marc
Chalancon, Guilhem
de Groot, Natalia S.
Wuster, Arthur
Babu, M. Madan
Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
title Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
title_full Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
title_fullStr Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
title_full_unstemmed Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
title_short Cells alter their tRNA abundance to selectively regulate protein synthesis during stress conditions
title_sort cells alter their trna abundance to selectively regulate protein synthesis during stress conditions
topic Stress Responses
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130803/
https://www.ncbi.nlm.nih.gov/pubmed/30181241
http://dx.doi.org/10.1126/scisignal.aat6409
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