Cargando…
Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine
Endothelium is the main source of catecholamine release in the electrical-field stimulation (EFS)–induced aortic contractions of the non- venomous snake Panterophis guttatus. However, adrenergic vasomotor control in venomous snakes such as Crotalus durissus terrificus and Bothrops jararaca has not y...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130875/ https://www.ncbi.nlm.nih.gov/pubmed/30199552 http://dx.doi.org/10.1371/journal.pone.0203573 |
_version_ | 1783354023449985024 |
---|---|
author | Campos, Rafael Justo, Alberto Fernando Oliveira Mónica, Fabíola Z. Cogo, José Carlos Moreno, Ronilson Agnaldo de Souza, Valéria Barbosa Schenka, Andre Almeida De Nucci, Gilberto |
author_facet | Campos, Rafael Justo, Alberto Fernando Oliveira Mónica, Fabíola Z. Cogo, José Carlos Moreno, Ronilson Agnaldo de Souza, Valéria Barbosa Schenka, Andre Almeida De Nucci, Gilberto |
author_sort | Campos, Rafael |
collection | PubMed |
description | Endothelium is the main source of catecholamine release in the electrical-field stimulation (EFS)–induced aortic contractions of the non- venomous snake Panterophis guttatus. However, adrenergic vasomotor control in venomous snakes such as Crotalus durissus terrificus and Bothrops jararaca has not yet been investigated. Crotalus and Bothrops aortic rings were mounted in an organ bath system. EFS-induced aortae contractions were performed in the presence and absence of guanethidine (30 μM), phentolamine (10 μM) or tetrodotoxin (1 μM). Frequency-induced contractions were also performed in aortae with endothelium removed. Immunohistochemical localization of both tyrosine hydroxylase (TH) and S-100 protein in snake aortic rings and brains, as well as in human tissue (paraganglioma tumour) were carried out. EFS (4 to 16 Hz) induced frequency-dependent aortic contractions in both Crotalus and Bothrops. The EFS-induced contractions were significantly reduced in the presence of either guanethidine or phentolamine in both snakes (p<0.05), whereas tetrodotoxin had no effect in either. Removal of the endothelium abolished the EFS-induced contractions in both snakes aortae (p<0.05). Immunohistochemistry revealed TH localization in endothelium of both snake aortae and human vessels. Nerve fibers were not observed in either snake aortae. In contrast, both TH and S100 protein were observed in snake brains and human tissue. Vascular endothelium is the main source of catecholamine release in EFS-induced contractions in Crotalus and Bothrops aortae. Human endothelial cells also expressed TH, indicating that endothelium- derived catecholamines possibly occur in mammalian vessels. |
format | Online Article Text |
id | pubmed-6130875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61308752018-09-15 Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine Campos, Rafael Justo, Alberto Fernando Oliveira Mónica, Fabíola Z. Cogo, José Carlos Moreno, Ronilson Agnaldo de Souza, Valéria Barbosa Schenka, Andre Almeida De Nucci, Gilberto PLoS One Research Article Endothelium is the main source of catecholamine release in the electrical-field stimulation (EFS)–induced aortic contractions of the non- venomous snake Panterophis guttatus. However, adrenergic vasomotor control in venomous snakes such as Crotalus durissus terrificus and Bothrops jararaca has not yet been investigated. Crotalus and Bothrops aortic rings were mounted in an organ bath system. EFS-induced aortae contractions were performed in the presence and absence of guanethidine (30 μM), phentolamine (10 μM) or tetrodotoxin (1 μM). Frequency-induced contractions were also performed in aortae with endothelium removed. Immunohistochemical localization of both tyrosine hydroxylase (TH) and S-100 protein in snake aortic rings and brains, as well as in human tissue (paraganglioma tumour) were carried out. EFS (4 to 16 Hz) induced frequency-dependent aortic contractions in both Crotalus and Bothrops. The EFS-induced contractions were significantly reduced in the presence of either guanethidine or phentolamine in both snakes (p<0.05), whereas tetrodotoxin had no effect in either. Removal of the endothelium abolished the EFS-induced contractions in both snakes aortae (p<0.05). Immunohistochemistry revealed TH localization in endothelium of both snake aortae and human vessels. Nerve fibers were not observed in either snake aortae. In contrast, both TH and S100 protein were observed in snake brains and human tissue. Vascular endothelium is the main source of catecholamine release in EFS-induced contractions in Crotalus and Bothrops aortae. Human endothelial cells also expressed TH, indicating that endothelium- derived catecholamines possibly occur in mammalian vessels. Public Library of Science 2018-09-10 /pmc/articles/PMC6130875/ /pubmed/30199552 http://dx.doi.org/10.1371/journal.pone.0203573 Text en © 2018 Campos et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Campos, Rafael Justo, Alberto Fernando Oliveira Mónica, Fabíola Z. Cogo, José Carlos Moreno, Ronilson Agnaldo de Souza, Valéria Barbosa Schenka, Andre Almeida De Nucci, Gilberto Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine |
title | Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine |
title_full | Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine |
title_fullStr | Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine |
title_full_unstemmed | Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine |
title_short | Electrical field-induced contractions on Crotalus durissus terrificus and Bothrops jararaca aortae are caused by endothelium-derived catecholamine |
title_sort | electrical field-induced contractions on crotalus durissus terrificus and bothrops jararaca aortae are caused by endothelium-derived catecholamine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130875/ https://www.ncbi.nlm.nih.gov/pubmed/30199552 http://dx.doi.org/10.1371/journal.pone.0203573 |
work_keys_str_mv | AT camposrafael electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT justoalbertofernandooliveira electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT monicafabiolaz electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT cogojosecarlos electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT morenoronilsonagnaldo electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT desouzavaleriabarbosa electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT schenkaandrealmeida electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine AT denuccigilberto electricalfieldinducedcontractionsoncrotalusdurissusterrificusandbothropsjararacaaortaearecausedbyendotheliumderivedcatecholamine |