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Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut

Ploidy-increasing cell cycles drive tissue growth in many developing organs. Such cycles, including endocycles, are increasingly appreciated to drive tissue growth following injury or activated growth signaling in mature organs. In these organs, the regulation and distinct roles of different cell cy...

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Detalles Bibliográficos
Autores principales: Cohen, Erez, Allen, Scott R, Sawyer, Jessica K, Fox, Donald T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130973/
https://www.ncbi.nlm.nih.gov/pubmed/30117808
http://dx.doi.org/10.7554/eLife.38327
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author Cohen, Erez
Allen, Scott R
Sawyer, Jessica K
Fox, Donald T
author_facet Cohen, Erez
Allen, Scott R
Sawyer, Jessica K
Fox, Donald T
author_sort Cohen, Erez
collection PubMed
description Ploidy-increasing cell cycles drive tissue growth in many developing organs. Such cycles, including endocycles, are increasingly appreciated to drive tissue growth following injury or activated growth signaling in mature organs. In these organs, the regulation and distinct roles of different cell cycles remains unclear. Here, we uncover a programmed switch between cell cycles in the Drosophila hindgut pylorus. Using an acute injury model, we identify mitosis as the response in larval pyloric cells, whereas endocycles occur in adult pyloric cells. By developing a novel genetic method, DEMISE (Dual-Expression-Method-for-Induced-Site-specific-Eradication), we show the cell cycle regulator Fizzy-related dictates the decision between mitosis and endocycles. After injury, both cycles accurately restore tissue mass and genome content. However, in response to sustained growth signaling, only endocycles preserve epithelial architecture. Our data reveal distinct cell cycle programming in response to similar stimuli in mature vs. developmental states and reveal a tissue-protective role of endocycles.
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spelling pubmed-61309732018-09-12 Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut Cohen, Erez Allen, Scott R Sawyer, Jessica K Fox, Donald T eLife Developmental Biology Ploidy-increasing cell cycles drive tissue growth in many developing organs. Such cycles, including endocycles, are increasingly appreciated to drive tissue growth following injury or activated growth signaling in mature organs. In these organs, the regulation and distinct roles of different cell cycles remains unclear. Here, we uncover a programmed switch between cell cycles in the Drosophila hindgut pylorus. Using an acute injury model, we identify mitosis as the response in larval pyloric cells, whereas endocycles occur in adult pyloric cells. By developing a novel genetic method, DEMISE (Dual-Expression-Method-for-Induced-Site-specific-Eradication), we show the cell cycle regulator Fizzy-related dictates the decision between mitosis and endocycles. After injury, both cycles accurately restore tissue mass and genome content. However, in response to sustained growth signaling, only endocycles preserve epithelial architecture. Our data reveal distinct cell cycle programming in response to similar stimuli in mature vs. developmental states and reveal a tissue-protective role of endocycles. eLife Sciences Publications, Ltd 2018-08-17 /pmc/articles/PMC6130973/ /pubmed/30117808 http://dx.doi.org/10.7554/eLife.38327 Text en © 2018, Cohen et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Cohen, Erez
Allen, Scott R
Sawyer, Jessica K
Fox, Donald T
Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut
title Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut
title_full Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut
title_fullStr Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut
title_full_unstemmed Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut
title_short Fizzy-Related dictates A cell cycle switch during organ repair and tissue growth responses in the Drosophila hindgut
title_sort fizzy-related dictates a cell cycle switch during organ repair and tissue growth responses in the drosophila hindgut
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130973/
https://www.ncbi.nlm.nih.gov/pubmed/30117808
http://dx.doi.org/10.7554/eLife.38327
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