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MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4
MiR-629-5p has been shown to function as a tumor promoter in some types of cancer. However, the role of miR-629-5p in colorectal cancer remains unclear. Here, the significant up-regulation of miR-629-5p in colorectal cancer tissues and cell lines was observed. Overexpression of miR-629-5p showed a p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131209/ https://www.ncbi.nlm.nih.gov/pubmed/30042169 http://dx.doi.org/10.1042/BSR20180613 |
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author | Lu, Jinlai Lu, Shuirong Li, Jingze Yu, Qi Liu, Lang Li, Qin |
author_facet | Lu, Jinlai Lu, Shuirong Li, Jingze Yu, Qi Liu, Lang Li, Qin |
author_sort | Lu, Jinlai |
collection | PubMed |
description | MiR-629-5p has been shown to function as a tumor promoter in some types of cancer. However, the role of miR-629-5p in colorectal cancer remains unclear. Here, the significant up-regulation of miR-629-5p in colorectal cancer tissues and cell lines was observed. Overexpression of miR-629-5p showed a positive effect on cell proliferation and migration. The enhanced miR-629-5p level also suppressed cell apoptosis and resulted in a low Bax level and a high Bcl-2 level. Further down-regulating miR-629-5p demonstrated opposite effects. CXXC finger protein 4 (CXXC4) was predicted as a direct target of miR-629-5p. Dual-luciferase reporter and Western blotting assays exhibited miR-629-5p directly bound to the 3′UTR of CXXC4 and then down-regulated its expression at post-transcriptional level. CXXC4 knockdown rescued the decreased cell proliferation and migration and the enhanced cell apoptosis induced by inhibiting miR-629-5p expression. Notably, overexpression of miR-629-5p also conferred 5-fluorouracil sensitivity, which was partly abrogated by coexpression of CXXC4. Overall, the results presented here suggest that miR-629-5p functions as a tumor promoter by improving proliferation and migration and repressing apoptosis and 5-FU sensitivity in colorectal cancer progression by directly down-regulating CXXC4. |
format | Online Article Text |
id | pubmed-6131209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61312092018-09-12 MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 Lu, Jinlai Lu, Shuirong Li, Jingze Yu, Qi Liu, Lang Li, Qin Biosci Rep Research Articles MiR-629-5p has been shown to function as a tumor promoter in some types of cancer. However, the role of miR-629-5p in colorectal cancer remains unclear. Here, the significant up-regulation of miR-629-5p in colorectal cancer tissues and cell lines was observed. Overexpression of miR-629-5p showed a positive effect on cell proliferation and migration. The enhanced miR-629-5p level also suppressed cell apoptosis and resulted in a low Bax level and a high Bcl-2 level. Further down-regulating miR-629-5p demonstrated opposite effects. CXXC finger protein 4 (CXXC4) was predicted as a direct target of miR-629-5p. Dual-luciferase reporter and Western blotting assays exhibited miR-629-5p directly bound to the 3′UTR of CXXC4 and then down-regulated its expression at post-transcriptional level. CXXC4 knockdown rescued the decreased cell proliferation and migration and the enhanced cell apoptosis induced by inhibiting miR-629-5p expression. Notably, overexpression of miR-629-5p also conferred 5-fluorouracil sensitivity, which was partly abrogated by coexpression of CXXC4. Overall, the results presented here suggest that miR-629-5p functions as a tumor promoter by improving proliferation and migration and repressing apoptosis and 5-FU sensitivity in colorectal cancer progression by directly down-regulating CXXC4. Portland Press Ltd. 2018-08-29 /pmc/articles/PMC6131209/ /pubmed/30042169 http://dx.doi.org/10.1042/BSR20180613 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Lu, Jinlai Lu, Shuirong Li, Jingze Yu, Qi Liu, Lang Li, Qin MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 |
title | MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 |
title_full | MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 |
title_fullStr | MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 |
title_full_unstemmed | MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 |
title_short | MiR-629-5p promotes colorectal cancer progression through targetting CXXC finger protein 4 |
title_sort | mir-629-5p promotes colorectal cancer progression through targetting cxxc finger protein 4 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131209/ https://www.ncbi.nlm.nih.gov/pubmed/30042169 http://dx.doi.org/10.1042/BSR20180613 |
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