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Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese

The mechanism of action by which oxidative stress induces granulosa cell apoptosis, which plays a vital role in initiating follicular atresia, is not well understood. In the present study, the effect of 3-nitropropionic acid (3-NPA) on oxidative stress and apoptosis in granulosa cells in geese was i...

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Autores principales: Kang, Bo, Wang, Xinxing, Xu, Qilin, Wu, Yongsheng, Si, Xiaohui, Jiang, Dongmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131328/
https://www.ncbi.nlm.nih.gov/pubmed/30042167
http://dx.doi.org/10.1042/BSR20180274
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author Kang, Bo
Wang, Xinxing
Xu, Qilin
Wu, Yongsheng
Si, Xiaohui
Jiang, Dongmei
author_facet Kang, Bo
Wang, Xinxing
Xu, Qilin
Wu, Yongsheng
Si, Xiaohui
Jiang, Dongmei
author_sort Kang, Bo
collection PubMed
description The mechanism of action by which oxidative stress induces granulosa cell apoptosis, which plays a vital role in initiating follicular atresia, is not well understood. In the present study, the effect of 3-nitropropionic acid (3-NPA) on oxidative stress and apoptosis in granulosa cells in geese was investigated. Our results showed that treatment with 3-NPA at 5.0 mmol/l for 24 h increased intracellular reactive oxygen species (ROS) production by 25.4% and decreased granulosa cell viability by 45.5% (P<0.05). Catalase and glutathione peroxidase gene expression levels in granulosa cells treated with 3-NPA were 1.32- and 0.49-fold compared with those of the control cells, respectively (P <0.05). A significant decrease in the expression level of B-cell lymphoma 2 (Bcl-2) protein and remarkable increases in the levels of Bax, p53 and cleaved-Caspase 3 proteins and the ratio of Bax/Bcl-2 expression in granulosa cells treated with 3-NPA were observed (P<0.05). Furthermore, a 38.43% increase in the percentage of early apoptotic cells was also observed in granulosa cells treated with 3-NPA (P<0.05). Moreover, the expression levels of NF-κB, Nrf2, Fhc, Hspa2 and Ho-1 in granulosa cells treated with 3-NPA were elevated 4.36-, 1.63-, 3.62-, 27.54- and 10.48-fold compared with those of the control cells (P<0.05), respectively. In conclusion, the present study demonstrates that treatment with 3-NPA induces ROS production and apoptosis and inhibits the viability of granulosa cells in geese. Furthermore, 3-NPA triggers increases in the expression of cleaved-Caspase 3 protein and the ratio of Bax/Bcl-2 expression, and induces the early apoptosis of granulosa cells.
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spelling pubmed-61313282018-09-12 Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese Kang, Bo Wang, Xinxing Xu, Qilin Wu, Yongsheng Si, Xiaohui Jiang, Dongmei Biosci Rep Research Articles The mechanism of action by which oxidative stress induces granulosa cell apoptosis, which plays a vital role in initiating follicular atresia, is not well understood. In the present study, the effect of 3-nitropropionic acid (3-NPA) on oxidative stress and apoptosis in granulosa cells in geese was investigated. Our results showed that treatment with 3-NPA at 5.0 mmol/l for 24 h increased intracellular reactive oxygen species (ROS) production by 25.4% and decreased granulosa cell viability by 45.5% (P<0.05). Catalase and glutathione peroxidase gene expression levels in granulosa cells treated with 3-NPA were 1.32- and 0.49-fold compared with those of the control cells, respectively (P <0.05). A significant decrease in the expression level of B-cell lymphoma 2 (Bcl-2) protein and remarkable increases in the levels of Bax, p53 and cleaved-Caspase 3 proteins and the ratio of Bax/Bcl-2 expression in granulosa cells treated with 3-NPA were observed (P<0.05). Furthermore, a 38.43% increase in the percentage of early apoptotic cells was also observed in granulosa cells treated with 3-NPA (P<0.05). Moreover, the expression levels of NF-κB, Nrf2, Fhc, Hspa2 and Ho-1 in granulosa cells treated with 3-NPA were elevated 4.36-, 1.63-, 3.62-, 27.54- and 10.48-fold compared with those of the control cells (P<0.05), respectively. In conclusion, the present study demonstrates that treatment with 3-NPA induces ROS production and apoptosis and inhibits the viability of granulosa cells in geese. Furthermore, 3-NPA triggers increases in the expression of cleaved-Caspase 3 protein and the ratio of Bax/Bcl-2 expression, and induces the early apoptosis of granulosa cells. Portland Press Ltd. 2018-09-12 /pmc/articles/PMC6131328/ /pubmed/30042167 http://dx.doi.org/10.1042/BSR20180274 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Kang, Bo
Wang, Xinxing
Xu, Qilin
Wu, Yongsheng
Si, Xiaohui
Jiang, Dongmei
Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
title Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
title_full Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
title_fullStr Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
title_full_unstemmed Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
title_short Effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
title_sort effect of 3-nitropropionic acid inducing oxidative stress and apoptosis of granulosa cells in geese
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131328/
https://www.ncbi.nlm.nih.gov/pubmed/30042167
http://dx.doi.org/10.1042/BSR20180274
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