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A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes

The myocardium behaves like a sophisticated orchestra that expresses its true potential only if each member performs the correct task harmonically. Recapitulating its complexity within engineered 3D functional constructs with tailored biological and mechanical properties, is one of the current scien...

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Autores principales: Maiullari, Fabio, Costantini, Marco, Milan, Marika, Pace, Valentina, Chirivì, Maila, Maiullari, Silvia, Rainer, Alberto, Baci, Denisa, Marei, Hany El-Sayed, Seliktar, Dror, Gargioli, Cesare, Bearzi, Claudia, Rizzi, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131510/
https://www.ncbi.nlm.nih.gov/pubmed/30201959
http://dx.doi.org/10.1038/s41598-018-31848-x
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author Maiullari, Fabio
Costantini, Marco
Milan, Marika
Pace, Valentina
Chirivì, Maila
Maiullari, Silvia
Rainer, Alberto
Baci, Denisa
Marei, Hany El-Sayed
Seliktar, Dror
Gargioli, Cesare
Bearzi, Claudia
Rizzi, Roberto
author_facet Maiullari, Fabio
Costantini, Marco
Milan, Marika
Pace, Valentina
Chirivì, Maila
Maiullari, Silvia
Rainer, Alberto
Baci, Denisa
Marei, Hany El-Sayed
Seliktar, Dror
Gargioli, Cesare
Bearzi, Claudia
Rizzi, Roberto
author_sort Maiullari, Fabio
collection PubMed
description The myocardium behaves like a sophisticated orchestra that expresses its true potential only if each member performs the correct task harmonically. Recapitulating its complexity within engineered 3D functional constructs with tailored biological and mechanical properties, is one of the current scientific priorities in the field of regenerative medicine and tissue engineering. In this study, driven by the necessity of fabricating advanced model of cardiac tissue, we present an innovative approach consisting of heterogeneous, multi-cellular constructs composed of Human Umbilical Vein Endothelial Cells (HUVECs) and induced pluripotent cell-derived cardiomyocytes (iPSC-CMs). Cells were encapsulated within hydrogel strands containing alginate and PEG-Fibrinogen (PF) and extruded through a custom microfluidic printing head (MPH) that allows to precisely tailor their 3D spatial deposition, guaranteeing a high printing fidelity and resolution. We obtained a 3D cardiac tissue compose of iPSC-derived CMs with a high orientation index imposed by the different defined geometries and blood vessel-like shapes generated by HUVECs which, as demonstrated by in vivo grafting, better support the integration of the engineered cardiac tissue with host’s vasculature.
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spelling pubmed-61315102018-09-13 A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes Maiullari, Fabio Costantini, Marco Milan, Marika Pace, Valentina Chirivì, Maila Maiullari, Silvia Rainer, Alberto Baci, Denisa Marei, Hany El-Sayed Seliktar, Dror Gargioli, Cesare Bearzi, Claudia Rizzi, Roberto Sci Rep Article The myocardium behaves like a sophisticated orchestra that expresses its true potential only if each member performs the correct task harmonically. Recapitulating its complexity within engineered 3D functional constructs with tailored biological and mechanical properties, is one of the current scientific priorities in the field of regenerative medicine and tissue engineering. In this study, driven by the necessity of fabricating advanced model of cardiac tissue, we present an innovative approach consisting of heterogeneous, multi-cellular constructs composed of Human Umbilical Vein Endothelial Cells (HUVECs) and induced pluripotent cell-derived cardiomyocytes (iPSC-CMs). Cells were encapsulated within hydrogel strands containing alginate and PEG-Fibrinogen (PF) and extruded through a custom microfluidic printing head (MPH) that allows to precisely tailor their 3D spatial deposition, guaranteeing a high printing fidelity and resolution. We obtained a 3D cardiac tissue compose of iPSC-derived CMs with a high orientation index imposed by the different defined geometries and blood vessel-like shapes generated by HUVECs which, as demonstrated by in vivo grafting, better support the integration of the engineered cardiac tissue with host’s vasculature. Nature Publishing Group UK 2018-09-10 /pmc/articles/PMC6131510/ /pubmed/30201959 http://dx.doi.org/10.1038/s41598-018-31848-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Maiullari, Fabio
Costantini, Marco
Milan, Marika
Pace, Valentina
Chirivì, Maila
Maiullari, Silvia
Rainer, Alberto
Baci, Denisa
Marei, Hany El-Sayed
Seliktar, Dror
Gargioli, Cesare
Bearzi, Claudia
Rizzi, Roberto
A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
title A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
title_full A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
title_fullStr A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
title_full_unstemmed A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
title_short A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
title_sort multi-cellular 3d bioprinting approach for vascularized heart tissue engineering based on huvecs and ipsc-derived cardiomyocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131510/
https://www.ncbi.nlm.nih.gov/pubmed/30201959
http://dx.doi.org/10.1038/s41598-018-31848-x
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