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Genetic diversity of Escherichia coli isolated from ice cube production sites

OBJECTIVE: The prevalence of Escherichia coli including from ice cubes in Indonesia is quite high. Unfortunately, little is known about the genetic diversity of E. coli from ice cube production site. Genotypic variation in E. coli populations is a major barrier to control public health risk associat...

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Autores principales: Pusparini, Novi, Waturangi, Diana E., Usia, Tepy, Nikastri, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131791/
https://www.ncbi.nlm.nih.gov/pubmed/30201028
http://dx.doi.org/10.1186/s13104-018-3737-3
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author Pusparini, Novi
Waturangi, Diana E.
Usia, Tepy
Nikastri, Eva
author_facet Pusparini, Novi
Waturangi, Diana E.
Usia, Tepy
Nikastri, Eva
author_sort Pusparini, Novi
collection PubMed
description OBJECTIVE: The prevalence of Escherichia coli including from ice cubes in Indonesia is quite high. Unfortunately, little is known about the genetic diversity of E. coli from ice cube production site. Genotypic variation in E. coli populations is a major barrier to control public health risk associated with foodborne pathogen. The aims of this study were to analyze the genotypic diversity of E. coli strains isolated from various samples in order to determine the genetic relationship between those strains. This study is also important to understand the occurrence, prevalence and profile picture of different pathogenic E. coli in various sources which potentially cause disease. RESULTS: Enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic polymerase chain reaction (REP-PCR) dendrogram showed high genetic diversity of 120 E. coli isolates in majority of sampling sites. DNA fingerprint patterns showed 26 and 21 clusters with 11 and 3 fingerprints individual lineages for ERIC and REP-PCR respectively. There was no correlation observed between phylogenetic relationship and virulence genes. The result indicated a variation of E. coli isolates in ice cube manufacturers. ERIC-PCR method is more discriminative compared with REP-PCR to analyze the genetic diversity of E. coli from ice cubes production sites.
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spelling pubmed-61317912018-09-13 Genetic diversity of Escherichia coli isolated from ice cube production sites Pusparini, Novi Waturangi, Diana E. Usia, Tepy Nikastri, Eva BMC Res Notes Research Note OBJECTIVE: The prevalence of Escherichia coli including from ice cubes in Indonesia is quite high. Unfortunately, little is known about the genetic diversity of E. coli from ice cube production site. Genotypic variation in E. coli populations is a major barrier to control public health risk associated with foodborne pathogen. The aims of this study were to analyze the genotypic diversity of E. coli strains isolated from various samples in order to determine the genetic relationship between those strains. This study is also important to understand the occurrence, prevalence and profile picture of different pathogenic E. coli in various sources which potentially cause disease. RESULTS: Enterobacterial repetitive intergenic consensus (ERIC) and repetitive extragenic palindromic polymerase chain reaction (REP-PCR) dendrogram showed high genetic diversity of 120 E. coli isolates in majority of sampling sites. DNA fingerprint patterns showed 26 and 21 clusters with 11 and 3 fingerprints individual lineages for ERIC and REP-PCR respectively. There was no correlation observed between phylogenetic relationship and virulence genes. The result indicated a variation of E. coli isolates in ice cube manufacturers. ERIC-PCR method is more discriminative compared with REP-PCR to analyze the genetic diversity of E. coli from ice cubes production sites. BioMed Central 2018-09-10 /pmc/articles/PMC6131791/ /pubmed/30201028 http://dx.doi.org/10.1186/s13104-018-3737-3 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Pusparini, Novi
Waturangi, Diana E.
Usia, Tepy
Nikastri, Eva
Genetic diversity of Escherichia coli isolated from ice cube production sites
title Genetic diversity of Escherichia coli isolated from ice cube production sites
title_full Genetic diversity of Escherichia coli isolated from ice cube production sites
title_fullStr Genetic diversity of Escherichia coli isolated from ice cube production sites
title_full_unstemmed Genetic diversity of Escherichia coli isolated from ice cube production sites
title_short Genetic diversity of Escherichia coli isolated from ice cube production sites
title_sort genetic diversity of escherichia coli isolated from ice cube production sites
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131791/
https://www.ncbi.nlm.nih.gov/pubmed/30201028
http://dx.doi.org/10.1186/s13104-018-3737-3
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