Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation

Asymmetrical flow field-flow fractionation (AF4) and hollow-fiber flow field-flow fractionation (HF5) are techniques widely used in analytical, industrial and biological analyses. The main problem in all AF4 and HF5 analyses is sample loss due to analyte–membrane interactions. In this work the impac...

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Detalles Bibliográficos
Autores principales: Kowalkowski, Tomasz, Sugajski, Mateusz, Buszewski, Bogusław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Original
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132554/
https://www.ncbi.nlm.nih.gov/pubmed/30220732
http://dx.doi.org/10.1007/s10337-018-3551-z
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author Kowalkowski, Tomasz
Sugajski, Mateusz
Buszewski, Bogusław
author_facet Kowalkowski, Tomasz
Sugajski, Mateusz
Buszewski, Bogusław
author_sort Kowalkowski, Tomasz
collection PubMed
description Asymmetrical flow field-flow fractionation (AF4) and hollow-fiber flow field-flow fractionation (HF5) are techniques widely used in analytical, industrial and biological analyses. The main problem in all AF4 and HF5 analyses is sample loss due to analyte–membrane interactions. In this work the impact of liquid carrier composition on latex nanoparticles (NPs) separation in water and two different concentrations of NH(4)NO(3) was studied. In AF4, a constant trend of decreasing the size of 60 and 121.9 nm particles induced by the ionic strength of the carrier liquid has been observed. In contrast, an agglomeration effect of the biggest 356 nm particles was observed when increasing ionic strength, which induced a significant drop of recovery to 35%. H5F provides better resolution and intensified peaks of NPs, but careful optimisation of system parameters is mandatory to obtain good separation.
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spelling pubmed-61325542018-09-14 Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation Kowalkowski, Tomasz Sugajski, Mateusz Buszewski, Bogusław Chromatographia Original Asymmetrical flow field-flow fractionation (AF4) and hollow-fiber flow field-flow fractionation (HF5) are techniques widely used in analytical, industrial and biological analyses. The main problem in all AF4 and HF5 analyses is sample loss due to analyte–membrane interactions. In this work the impact of liquid carrier composition on latex nanoparticles (NPs) separation in water and two different concentrations of NH(4)NO(3) was studied. In AF4, a constant trend of decreasing the size of 60 and 121.9 nm particles induced by the ionic strength of the carrier liquid has been observed. In contrast, an agglomeration effect of the biggest 356 nm particles was observed when increasing ionic strength, which induced a significant drop of recovery to 35%. H5F provides better resolution and intensified peaks of NPs, but careful optimisation of system parameters is mandatory to obtain good separation. Springer Berlin Heidelberg 2018-06-14 2018 /pmc/articles/PMC6132554/ /pubmed/30220732 http://dx.doi.org/10.1007/s10337-018-3551-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original
Kowalkowski, Tomasz
Sugajski, Mateusz
Buszewski, Bogusław
Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation
title Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation
title_full Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation
title_fullStr Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation
title_full_unstemmed Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation
title_short Impact of Ionic Strength of Carrier Liquid on Recovery in Flow Field-Flow Fractionation
title_sort impact of ionic strength of carrier liquid on recovery in flow field-flow fractionation
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132554/
https://www.ncbi.nlm.nih.gov/pubmed/30220732
http://dx.doi.org/10.1007/s10337-018-3551-z
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AT sugajskimateusz impactofionicstrengthofcarrierliquidonrecoveryinflowfieldflowfractionation
AT buszewskibogusław impactofionicstrengthofcarrierliquidonrecoveryinflowfieldflowfractionation

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