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Of mice and men: the host response to influenza virus infection

Influenza virus (IV) infections represent a very serious public health problem. At present, no established biomarkers exist to support diagnosis for respiratory viral infections and more importantly for severe IV disease. Studies in animal models are extremely important to understand the biological,...

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Autores principales: Kollmus, Heike, Pilzner, Carolin, Leist, Sarah R., Heise, Mark, Geffers, Robert, Schughart, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132725/
https://www.ncbi.nlm.nih.gov/pubmed/29947965
http://dx.doi.org/10.1007/s00335-018-9750-y
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author Kollmus, Heike
Pilzner, Carolin
Leist, Sarah R.
Heise, Mark
Geffers, Robert
Schughart, Klaus
author_facet Kollmus, Heike
Pilzner, Carolin
Leist, Sarah R.
Heise, Mark
Geffers, Robert
Schughart, Klaus
author_sort Kollmus, Heike
collection PubMed
description Influenza virus (IV) infections represent a very serious public health problem. At present, no established biomarkers exist to support diagnosis for respiratory viral infections and more importantly for severe IV disease. Studies in animal models are extremely important to understand the biological, genetic, and environmental factors that contribute to severe IV disease and to validate biomarker candidates from human studies. However, mouse human cross-species comparisons are often compromised by the fact that animal studies concentrate on the infected lungs, whereas in humans almost all studies use peripheral blood from patients. In addition, human studies do not consider genetic background as variable although human populations are genetically very diverse. Therefore, in this study, we performed a cross-species gene expression study of the peripheral blood from human patients and from the highly genetically diverse Collaborative Cross (CC) mouse population after IV infection. Our results demonstrate that changes of gene expression in individual genes are highly similar in mice and humans. The top-regulated genes in humans were also differentially regulated in mice. We conclude that the mouse is a highly valuable in vivo model system to validate and to discover gene candidates which can be used as biomarkers in humans. Furthermore, mouse studies allow confirmation of findings in humans in a well-controlled experimental system adding enormous value to the understanding of expression and function of human candidate genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00335-018-9750-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-61327252018-09-13 Of mice and men: the host response to influenza virus infection Kollmus, Heike Pilzner, Carolin Leist, Sarah R. Heise, Mark Geffers, Robert Schughart, Klaus Mamm Genome Article Influenza virus (IV) infections represent a very serious public health problem. At present, no established biomarkers exist to support diagnosis for respiratory viral infections and more importantly for severe IV disease. Studies in animal models are extremely important to understand the biological, genetic, and environmental factors that contribute to severe IV disease and to validate biomarker candidates from human studies. However, mouse human cross-species comparisons are often compromised by the fact that animal studies concentrate on the infected lungs, whereas in humans almost all studies use peripheral blood from patients. In addition, human studies do not consider genetic background as variable although human populations are genetically very diverse. Therefore, in this study, we performed a cross-species gene expression study of the peripheral blood from human patients and from the highly genetically diverse Collaborative Cross (CC) mouse population after IV infection. Our results demonstrate that changes of gene expression in individual genes are highly similar in mice and humans. The top-regulated genes in humans were also differentially regulated in mice. We conclude that the mouse is a highly valuable in vivo model system to validate and to discover gene candidates which can be used as biomarkers in humans. Furthermore, mouse studies allow confirmation of findings in humans in a well-controlled experimental system adding enormous value to the understanding of expression and function of human candidate genes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00335-018-9750-y) contains supplementary material, which is available to authorized users. Springer US 2018-06-15 2018 /pmc/articles/PMC6132725/ /pubmed/29947965 http://dx.doi.org/10.1007/s00335-018-9750-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Kollmus, Heike
Pilzner, Carolin
Leist, Sarah R.
Heise, Mark
Geffers, Robert
Schughart, Klaus
Of mice and men: the host response to influenza virus infection
title Of mice and men: the host response to influenza virus infection
title_full Of mice and men: the host response to influenza virus infection
title_fullStr Of mice and men: the host response to influenza virus infection
title_full_unstemmed Of mice and men: the host response to influenza virus infection
title_short Of mice and men: the host response to influenza virus infection
title_sort of mice and men: the host response to influenza virus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132725/
https://www.ncbi.nlm.nih.gov/pubmed/29947965
http://dx.doi.org/10.1007/s00335-018-9750-y
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