Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032

The genomes of two new lytic phages of Corynebacterium glutamicum ATCC 13032, φ673 and φ674, were sequenced and annotated (GenBank: MG324353, MG324354). Electron microscopy studies of both virions revealed that taxonomically they belong to the Siphoviridae family and have a polyhedral head with a wi...

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Autores principales: Yomantas, Yurgis A. V., Abalakina, Elena G., Lobanova, Juliya S., Mamontov, Victor A., Stoynova, Nataliya V., Mashko, Sergey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132909/
https://www.ncbi.nlm.nih.gov/pubmed/29766331
http://dx.doi.org/10.1007/s00705-018-3867-x
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author Yomantas, Yurgis A. V.
Abalakina, Elena G.
Lobanova, Juliya S.
Mamontov, Victor A.
Stoynova, Nataliya V.
Mashko, Sergey V.
author_facet Yomantas, Yurgis A. V.
Abalakina, Elena G.
Lobanova, Juliya S.
Mamontov, Victor A.
Stoynova, Nataliya V.
Mashko, Sergey V.
author_sort Yomantas, Yurgis A. V.
collection PubMed
description The genomes of two new lytic phages of Corynebacterium glutamicum ATCC 13032, φ673 and φ674, were sequenced and annotated (GenBank: MG324353, MG324354). Electron microscopy studies of both virions revealed that taxonomically they belong to the Siphoviridae family and have a polyhedral head with a width of 50 nm and a non-contractile tail with a length of 250 nm. The genomes of φ673 and φ674 consist of linear double-stranded DNA molecules with lengths of 44,530 bp (G+C = 51.1%) and 43,193 bp (G+C = 50.7%) and identical, protruding, cohesive 3’ ends 13 nt in length. The level of identity between the φ673 and φ674 genomes is 85.2%. Two major structural proteins of each virion were separated via SDS-PAGE and identified using peptide mass fingerprinting. Based on bioinformatic analysis, 56 and 54 ORFs were predicted for φ673 and φ674, respectively. Only 20 of the putative gene products of φ673 and 20 of φ674 could be assigned to known functions. Both genomes were divided into functional modules. Nine putative promoters in the φ673 genome and eight in the φ674 genome were predicted. One bidirectional Rho-independent transcription terminator was identified and experimentally confirmed in each phage genome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-018-3867-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-61329092018-09-13 Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032 Yomantas, Yurgis A. V. Abalakina, Elena G. Lobanova, Juliya S. Mamontov, Victor A. Stoynova, Nataliya V. Mashko, Sergey V. Arch Virol Annotated Sequence Record The genomes of two new lytic phages of Corynebacterium glutamicum ATCC 13032, φ673 and φ674, were sequenced and annotated (GenBank: MG324353, MG324354). Electron microscopy studies of both virions revealed that taxonomically they belong to the Siphoviridae family and have a polyhedral head with a width of 50 nm and a non-contractile tail with a length of 250 nm. The genomes of φ673 and φ674 consist of linear double-stranded DNA molecules with lengths of 44,530 bp (G+C = 51.1%) and 43,193 bp (G+C = 50.7%) and identical, protruding, cohesive 3’ ends 13 nt in length. The level of identity between the φ673 and φ674 genomes is 85.2%. Two major structural proteins of each virion were separated via SDS-PAGE and identified using peptide mass fingerprinting. Based on bioinformatic analysis, 56 and 54 ORFs were predicted for φ673 and φ674, respectively. Only 20 of the putative gene products of φ673 and 20 of φ674 could be assigned to known functions. Both genomes were divided into functional modules. Nine putative promoters in the φ673 genome and eight in the φ674 genome were predicted. One bidirectional Rho-independent transcription terminator was identified and experimentally confirmed in each phage genome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-018-3867-x) contains supplementary material, which is available to authorized users. Springer Vienna 2018-05-15 2018 /pmc/articles/PMC6132909/ /pubmed/29766331 http://dx.doi.org/10.1007/s00705-018-3867-x Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Annotated Sequence Record
Yomantas, Yurgis A. V.
Abalakina, Elena G.
Lobanova, Juliya S.
Mamontov, Victor A.
Stoynova, Nataliya V.
Mashko, Sergey V.
Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032
title Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032
title_full Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032
title_fullStr Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032
title_full_unstemmed Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032
title_short Complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of Corynebacterium glutamicum ATCC 13032
title_sort complete nucleotide sequences and annotations of φ673 and φ674, two newly characterised lytic phages of corynebacterium glutamicum atcc 13032
topic Annotated Sequence Record
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132909/
https://www.ncbi.nlm.nih.gov/pubmed/29766331
http://dx.doi.org/10.1007/s00705-018-3867-x
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