FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa

The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2n = 2x = 16; Aconitum variegatum, A. degenii) and tetraploids (2n = 4× = 32; A. firmum, A. plicatum), rDNA repeats were l...

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Autores principales: Joachimiak, Andrzej J., Hasterok, Robert, Sliwinska, Elwira, Musiał, Krystyna, Grabowska-Joachimiak, Aleksandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133112/
https://www.ncbi.nlm.nih.gov/pubmed/29541843
http://dx.doi.org/10.1007/s00709-018-1238-9
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author Joachimiak, Andrzej J.
Hasterok, Robert
Sliwinska, Elwira
Musiał, Krystyna
Grabowska-Joachimiak, Aleksandra
author_facet Joachimiak, Andrzej J.
Hasterok, Robert
Sliwinska, Elwira
Musiał, Krystyna
Grabowska-Joachimiak, Aleksandra
author_sort Joachimiak, Andrzej J.
collection PubMed
description The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2n = 2x = 16; Aconitum variegatum, A. degenii) and tetraploids (2n = 4× = 32; A. firmum, A. plicatum), rDNA repeats were localized exclusively on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All analyzed species showed similar basal genome size (Cx = 5.31–5.71 pg). The most striking features of tetraploid karyotypes were the conservation of diploid rDNA loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution of excessive ribosomal sites suggests their role in the secondary diploidization of tetraploid karyotypes.
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spelling pubmed-61331122018-09-18 FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa Joachimiak, Andrzej J. Hasterok, Robert Sliwinska, Elwira Musiał, Krystyna Grabowska-Joachimiak, Aleksandra Protoplasma Original Article The location of 5S and 35S rDNA sequences in chromosomes of four Aconitum subsp. Aconitum species was analyzed after fluorescence in situ hybridization (FISH). Both in diploids (2n = 2x = 16; Aconitum variegatum, A. degenii) and tetraploids (2n = 4× = 32; A. firmum, A. plicatum), rDNA repeats were localized exclusively on the shorter arms of chromosomes, in subterminal or pericentromeric sites. All analyzed species showed similar basal genome size (Cx = 5.31–5.71 pg). The most striking features of tetraploid karyotypes were the conservation of diploid rDNA loci and emergence of many additional 5S rDNA clusters. Chromosomal distribution of excessive ribosomal sites suggests their role in the secondary diploidization of tetraploid karyotypes. Springer Vienna 2018-03-14 2018 /pmc/articles/PMC6133112/ /pubmed/29541843 http://dx.doi.org/10.1007/s00709-018-1238-9 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Joachimiak, Andrzej J.
Hasterok, Robert
Sliwinska, Elwira
Musiał, Krystyna
Grabowska-Joachimiak, Aleksandra
FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa
title FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa
title_full FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa
title_fullStr FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa
title_full_unstemmed FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa
title_short FISH-aimed karyotype analysis in Aconitum subgen. Aconitum reveals excessive rDNA sites in tetraploid taxa
title_sort fish-aimed karyotype analysis in aconitum subgen. aconitum reveals excessive rdna sites in tetraploid taxa
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133112/
https://www.ncbi.nlm.nih.gov/pubmed/29541843
http://dx.doi.org/10.1007/s00709-018-1238-9
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