Cargando…
Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Si...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133545/ https://www.ncbi.nlm.nih.gov/pubmed/30201094 http://dx.doi.org/10.7554/eLife.37295 |
_version_ | 1783354536946040832 |
---|---|
author | Jiménez-Zaragoza, Manuel Yubero, Marina PL Martín-Forero, Esther Castón, Jose R Reguera, David Luque, Daniel de Pablo, Pedro J Rodríguez, Javier M |
author_facet | Jiménez-Zaragoza, Manuel Yubero, Marina PL Martín-Forero, Esther Castón, Jose R Reguera, David Luque, Daniel de Pablo, Pedro J Rodríguez, Javier M |
author_sort | Jiménez-Zaragoza, Manuel |
collection | PubMed |
description | The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion. |
format | Online Article Text |
id | pubmed-6133545 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-61335452018-09-12 Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus Jiménez-Zaragoza, Manuel Yubero, Marina PL Martín-Forero, Esther Castón, Jose R Reguera, David Luque, Daniel de Pablo, Pedro J Rodríguez, Javier M eLife Physics of Living Systems The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion. eLife Sciences Publications, Ltd 2018-09-11 /pmc/articles/PMC6133545/ /pubmed/30201094 http://dx.doi.org/10.7554/eLife.37295 Text en © 2018, Jiménez-Zaragoza et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Physics of Living Systems Jiménez-Zaragoza, Manuel Yubero, Marina PL Martín-Forero, Esther Castón, Jose R Reguera, David Luque, Daniel de Pablo, Pedro J Rodríguez, Javier M Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
title | Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
title_full | Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
title_fullStr | Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
title_full_unstemmed | Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
title_short | Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
title_sort | biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus |
topic | Physics of Living Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133545/ https://www.ncbi.nlm.nih.gov/pubmed/30201094 http://dx.doi.org/10.7554/eLife.37295 |
work_keys_str_mv | AT jimenezzaragozamanuel biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT yuberomarinapl biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT martinforeroesther biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT castonjoser biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT regueradavid biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT luquedaniel biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT depablopedroj biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus AT rodriguezjavierm biophysicalpropertiesofsinglerotavirusparticlesaccountforthefunctionsofproteinshellsinamultilayeredvirus |