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A carbon nanotube integrated microfluidic device for blood plasma extraction

Blood is a complex fluid consisting of cells and plasma. Plasma contains key biomarkers essential for disease diagnosis and therapeutic monitoring. Thus, by separating plasma from the blood, it is possible to analyze these biomarkers. Conventional methods for plasma extraction involve bulky equipmen...

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Autores principales: Yeh, Yin-Ting, Lin, Zhong, Zheng, Si-Yang, Terrones, Mauricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133936/
https://www.ncbi.nlm.nih.gov/pubmed/30206295
http://dx.doi.org/10.1038/s41598-018-31810-x
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author Yeh, Yin-Ting
Lin, Zhong
Zheng, Si-Yang
Terrones, Mauricio
author_facet Yeh, Yin-Ting
Lin, Zhong
Zheng, Si-Yang
Terrones, Mauricio
author_sort Yeh, Yin-Ting
collection PubMed
description Blood is a complex fluid consisting of cells and plasma. Plasma contains key biomarkers essential for disease diagnosis and therapeutic monitoring. Thus, by separating plasma from the blood, it is possible to analyze these biomarkers. Conventional methods for plasma extraction involve bulky equipment, and miniaturization constitutes a key step to develop portable devices for plasma extraction. Here, we integrated nanomaterial synthesis with microfabrication, and built a microfluidic device. In particular, we designed a double-spiral channel able to perform cross-flow filtration. This channel was constructed by growing aligned carbon nanotubes (CNTs) with average inter-tubular distances of ~80 nm, which resulted in porosity values of ~93%. During blood extraction, these aligned CNTs allow smaller molecules (e.g., proteins) to pass through the channel wall, while larger molecules (e.g., cells) get blocked. Our results show that our device effectively separates plasma from blood, by trapping blood cells. We successfully recovered albumin -the most abundant protein inside plasma- with an efficiency of ~80%. This work constitutes the first report on integrating biocompatible nitrogen-doped CNT (CN(x)CNT) arrays to extract plasma from human blood, thus widening the bio-applications of CNTs.
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spelling pubmed-61339362018-09-15 A carbon nanotube integrated microfluidic device for blood plasma extraction Yeh, Yin-Ting Lin, Zhong Zheng, Si-Yang Terrones, Mauricio Sci Rep Article Blood is a complex fluid consisting of cells and plasma. Plasma contains key biomarkers essential for disease diagnosis and therapeutic monitoring. Thus, by separating plasma from the blood, it is possible to analyze these biomarkers. Conventional methods for plasma extraction involve bulky equipment, and miniaturization constitutes a key step to develop portable devices for plasma extraction. Here, we integrated nanomaterial synthesis with microfabrication, and built a microfluidic device. In particular, we designed a double-spiral channel able to perform cross-flow filtration. This channel was constructed by growing aligned carbon nanotubes (CNTs) with average inter-tubular distances of ~80 nm, which resulted in porosity values of ~93%. During blood extraction, these aligned CNTs allow smaller molecules (e.g., proteins) to pass through the channel wall, while larger molecules (e.g., cells) get blocked. Our results show that our device effectively separates plasma from blood, by trapping blood cells. We successfully recovered albumin -the most abundant protein inside plasma- with an efficiency of ~80%. This work constitutes the first report on integrating biocompatible nitrogen-doped CNT (CN(x)CNT) arrays to extract plasma from human blood, thus widening the bio-applications of CNTs. Nature Publishing Group UK 2018-09-11 /pmc/articles/PMC6133936/ /pubmed/30206295 http://dx.doi.org/10.1038/s41598-018-31810-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yeh, Yin-Ting
Lin, Zhong
Zheng, Si-Yang
Terrones, Mauricio
A carbon nanotube integrated microfluidic device for blood plasma extraction
title A carbon nanotube integrated microfluidic device for blood plasma extraction
title_full A carbon nanotube integrated microfluidic device for blood plasma extraction
title_fullStr A carbon nanotube integrated microfluidic device for blood plasma extraction
title_full_unstemmed A carbon nanotube integrated microfluidic device for blood plasma extraction
title_short A carbon nanotube integrated microfluidic device for blood plasma extraction
title_sort carbon nanotube integrated microfluidic device for blood plasma extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133936/
https://www.ncbi.nlm.nih.gov/pubmed/30206295
http://dx.doi.org/10.1038/s41598-018-31810-x
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