A new hybrid approach for MHC genotyping: high-throughput NGS and long read MinION nanopore sequencing, with application to the non-model vertebrate Alpine chamois (Rupicapra rupicapra)

The major histocompatibility complex (MHC) acts as an interface between the immune system and infectious diseases. Accurate characterization and genotyping of the extremely variable MHC loci are challenging especially without a reference sequence. We designed a combination of long-range PCR, Illumin...

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Detalles Bibliográficos
Autores principales: Fuselli, S., Baptista, R. P., Panziera, A., Magi, A., Guglielmi, S., Tonin, R., Benazzo, A., Bauzer, L. G., Mazzoni, C. J., Bertorelle, G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133961/
https://www.ncbi.nlm.nih.gov/pubmed/29572469
http://dx.doi.org/10.1038/s41437-018-0070-5

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