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New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)

Sex reversal in insects, amphibians, reptiles, and fishes is a complicated and interesting biological phenomenon. Sex reversal changes the sex ratio of populations and may complicate breeding schemes. In the Chinese tongue sole (Cynoglossus semilaevis), genetic females may change into pseudomales, t...

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Autores principales: Cui, Yu, Wang, Weifeng, Ma, Liyong, Jie, Jinhua, Zhang, Yanhong, Wang, Huanling, Li, Hengde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134077/
https://www.ncbi.nlm.nih.gov/pubmed/30093666
http://dx.doi.org/10.1038/s41437-018-0126-6
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author Cui, Yu
Wang, Weifeng
Ma, Liyong
Jie, Jinhua
Zhang, Yanhong
Wang, Huanling
Li, Hengde
author_facet Cui, Yu
Wang, Weifeng
Ma, Liyong
Jie, Jinhua
Zhang, Yanhong
Wang, Huanling
Li, Hengde
author_sort Cui, Yu
collection PubMed
description Sex reversal in insects, amphibians, reptiles, and fishes is a complicated and interesting biological phenomenon. Sex reversal changes the sex ratio of populations and may complicate breeding schemes. In the Chinese tongue sole (Cynoglossus semilaevis), genetic females may change into pseudomales, thereby increasing aquaculture costs because of the lower growth rate of the males than that of the females. Here we identify a new locus associated with sex reversal; this single nucleotide polymorphism (SNP) is located in the third intron of the doublesex and mab-3 related transcription factor 1 (Dmrt1) gene on the Z chromosome (named Cyn_Z_8564889) and has two alleles, A and G. Cyn_Z_8564889 regulates sex reversal interactively with our previously detected SNP (Cyn_Z_6676874), with the genetic females simultaneously carrying the T allele of Cyn_Z_6676874 and the A allele of Cyn_Z_8564889 changing into pseudomales. Other Dmrt1 polymorphisms were detected, which formed two haplotypes. Two SNPs in the second exon of Dmrt1 result in amino acid changes, suggesting that Dmrt1 is essential in sex reversal. We also verified that pseudomales produce no or little W sperm. The interaction and linkage between Cyn_Z_6676874 and Cyn_Z_8564889 and the absence of W sperm from pseudomales unravel the genetic architecture of sex reversal in C. semilaevis.
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spelling pubmed-61340772018-09-13 New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis) Cui, Yu Wang, Weifeng Ma, Liyong Jie, Jinhua Zhang, Yanhong Wang, Huanling Li, Hengde Heredity (Edinb) Article Sex reversal in insects, amphibians, reptiles, and fishes is a complicated and interesting biological phenomenon. Sex reversal changes the sex ratio of populations and may complicate breeding schemes. In the Chinese tongue sole (Cynoglossus semilaevis), genetic females may change into pseudomales, thereby increasing aquaculture costs because of the lower growth rate of the males than that of the females. Here we identify a new locus associated with sex reversal; this single nucleotide polymorphism (SNP) is located in the third intron of the doublesex and mab-3 related transcription factor 1 (Dmrt1) gene on the Z chromosome (named Cyn_Z_8564889) and has two alleles, A and G. Cyn_Z_8564889 regulates sex reversal interactively with our previously detected SNP (Cyn_Z_6676874), with the genetic females simultaneously carrying the T allele of Cyn_Z_6676874 and the A allele of Cyn_Z_8564889 changing into pseudomales. Other Dmrt1 polymorphisms were detected, which formed two haplotypes. Two SNPs in the second exon of Dmrt1 result in amino acid changes, suggesting that Dmrt1 is essential in sex reversal. We also verified that pseudomales produce no or little W sperm. The interaction and linkage between Cyn_Z_6676874 and Cyn_Z_8564889 and the absence of W sperm from pseudomales unravel the genetic architecture of sex reversal in C. semilaevis. Springer International Publishing 2018-08-09 2018-10 /pmc/articles/PMC6134077/ /pubmed/30093666 http://dx.doi.org/10.1038/s41437-018-0126-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Cui, Yu
Wang, Weifeng
Ma, Liyong
Jie, Jinhua
Zhang, Yanhong
Wang, Huanling
Li, Hengde
New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)
title New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)
title_full New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)
title_fullStr New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)
title_full_unstemmed New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)
title_short New locus reveals the genetic architecture of sex reversal in the Chinese tongue sole (Cynoglossus semilaevis)
title_sort new locus reveals the genetic architecture of sex reversal in the chinese tongue sole (cynoglossus semilaevis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134077/
https://www.ncbi.nlm.nih.gov/pubmed/30093666
http://dx.doi.org/10.1038/s41437-018-0126-6
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