Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)

This data article contains descriptive and experimental data on the construction, expression, and simple characterization of AG1(1–843) and AG1(1–1581). AG1 is an important member of the DUF1220 protein family. It׳s hard to get the recombinant protein because of its DNA sequence. The DNA sequence we...

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Detalles Bibliográficos
Autores principales: Yan, Xie, Yang, Yan-Tao, Shi, Wei, Ai, Xia, Xi, Xu-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Physics and Astronomy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134160/
https://www.ncbi.nlm.nih.gov/pubmed/30211279
http://dx.doi.org/10.1016/j.dib.2018.08.094
Descripción
Sumario:This data article contains descriptive and experimental data on the construction, expression, and simple characterization of AG1(1–843) and AG1(1–1581). AG1 is an important member of the DUF1220 protein family. It׳s hard to get the recombinant protein because of its DNA sequence. The DNA sequence were optimized by proper design, cloned by overlap PCR and constructed into expression vector. AG1(1–843) and AG1(1–1581).were over expressed in Escherichia coli, purified and analyzed by dynamic light scattering and gel filtration analysis. An effective technique is provided to construct and express proteins with complicated sequences.

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