Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)

This data article contains descriptive and experimental data on the construction, expression, and simple characterization of AG1(1–843) and AG1(1–1581). AG1 is an important member of the DUF1220 protein family. It׳s hard to get the recombinant protein because of its DNA sequence. The DNA sequence we...

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Detalles Bibliográficos
Autores principales: Yan, Xie, Yang, Yan-Tao, Shi, Wei, Ai, Xia, Xi, Xu-Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Physics and Astronomy
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134160/
https://www.ncbi.nlm.nih.gov/pubmed/30211279
http://dx.doi.org/10.1016/j.dib.2018.08.094
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author Yan, Xie
Yang, Yan-Tao
Shi, Wei
Ai, Xia
Xi, Xu-Guang
author_facet Yan, Xie
Yang, Yan-Tao
Shi, Wei
Ai, Xia
Xi, Xu-Guang
author_sort Yan, Xie
collection PubMed
description This data article contains descriptive and experimental data on the construction, expression, and simple characterization of AG1(1–843) and AG1(1–1581). AG1 is an important member of the DUF1220 protein family. It׳s hard to get the recombinant protein because of its DNA sequence. The DNA sequence were optimized by proper design, cloned by overlap PCR and constructed into expression vector. AG1(1–843) and AG1(1–1581).were over expressed in Escherichia coli, purified and analyzed by dynamic light scattering and gel filtration analysis. An effective technique is provided to construct and express proteins with complicated sequences.
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spelling pubmed-61341602018-09-12 Construction, expression, and characterization of AG1(1–843) and AG1(1–1581) Yan, Xie Yang, Yan-Tao Shi, Wei Ai, Xia Xi, Xu-Guang Data Brief Physics and Astronomy This data article contains descriptive and experimental data on the construction, expression, and simple characterization of AG1(1–843) and AG1(1–1581). AG1 is an important member of the DUF1220 protein family. It׳s hard to get the recombinant protein because of its DNA sequence. The DNA sequence were optimized by proper design, cloned by overlap PCR and constructed into expression vector. AG1(1–843) and AG1(1–1581).were over expressed in Escherichia coli, purified and analyzed by dynamic light scattering and gel filtration analysis. An effective technique is provided to construct and express proteins with complicated sequences. Elsevier 2018-08-30 /pmc/articles/PMC6134160/ /pubmed/30211279 http://dx.doi.org/10.1016/j.dib.2018.08.094 Text en © 2018 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Physics and Astronomy
Yan, Xie
Yang, Yan-Tao
Shi, Wei
Ai, Xia
Xi, Xu-Guang
Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)
title Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)
title_full Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)
title_fullStr Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)
title_full_unstemmed Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)
title_short Construction, expression, and characterization of AG1(1–843) and AG1(1–1581)
title_sort construction, expression, and characterization of ag1(1–843) and ag1(1–1581)
topic Physics and Astronomy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134160/
https://www.ncbi.nlm.nih.gov/pubmed/30211279
http://dx.doi.org/10.1016/j.dib.2018.08.094
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