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Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report

BACKGROUND: We report on a female patient who underwent primary radical resection for a stage 2B Her-2-positive Barrett’s-type esophageal adenocarcinoma (EAC). Despite Her-2 targeted therapy, her disease recurred and required repeated metastectomies. CASE PRESENTATION: Digital cell sorting and targe...

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Autores principales: Isidori, Federica, Malvi, Deborah, Fittipaldi, Silvia, Forcato, Claudio, Bozzarelli, Isotta, Sala, Claudia, Raulli, Giovanni, D’Errico, Antonia, Fiorentino, Michelangelo, Seri, Marco, Krishnadath, Kausilia K., Bonora, Elena, Mattioli, Sandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134594/
https://www.ncbi.nlm.nih.gov/pubmed/30208867
http://dx.doi.org/10.1186/s12885-018-4789-4
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author Isidori, Federica
Malvi, Deborah
Fittipaldi, Silvia
Forcato, Claudio
Bozzarelli, Isotta
Sala, Claudia
Raulli, Giovanni
D’Errico, Antonia
Fiorentino, Michelangelo
Seri, Marco
Krishnadath, Kausilia K.
Bonora, Elena
Mattioli, Sandro
author_facet Isidori, Federica
Malvi, Deborah
Fittipaldi, Silvia
Forcato, Claudio
Bozzarelli, Isotta
Sala, Claudia
Raulli, Giovanni
D’Errico, Antonia
Fiorentino, Michelangelo
Seri, Marco
Krishnadath, Kausilia K.
Bonora, Elena
Mattioli, Sandro
author_sort Isidori, Federica
collection PubMed
description BACKGROUND: We report on a female patient who underwent primary radical resection for a stage 2B Her-2-positive Barrett’s-type esophageal adenocarcinoma (EAC). Despite Her-2 targeted therapy, her disease recurred and required repeated metastectomies. CASE PRESENTATION: Digital cell sorting and targeted sequencing of cancer sub-clones from EAC and metastases revealed a completely mutated TP53, whereas the sorted stromal cells were wild-type. Her-2 amplification was significantly lower in the metastases when the patient became therapy-resistant. CONCLUSIONS: The mechanism of therapy resistance illustrated by this case could only be detected through accurate analysis of tumor sub-populations. Investigating tumor sub-populations of recurrent disease is important for adjusting therapy in recurrent EAC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-018-4789-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-61345942018-09-13 Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report Isidori, Federica Malvi, Deborah Fittipaldi, Silvia Forcato, Claudio Bozzarelli, Isotta Sala, Claudia Raulli, Giovanni D’Errico, Antonia Fiorentino, Michelangelo Seri, Marco Krishnadath, Kausilia K. Bonora, Elena Mattioli, Sandro BMC Cancer Case Report BACKGROUND: We report on a female patient who underwent primary radical resection for a stage 2B Her-2-positive Barrett’s-type esophageal adenocarcinoma (EAC). Despite Her-2 targeted therapy, her disease recurred and required repeated metastectomies. CASE PRESENTATION: Digital cell sorting and targeted sequencing of cancer sub-clones from EAC and metastases revealed a completely mutated TP53, whereas the sorted stromal cells were wild-type. Her-2 amplification was significantly lower in the metastases when the patient became therapy-resistant. CONCLUSIONS: The mechanism of therapy resistance illustrated by this case could only be detected through accurate analysis of tumor sub-populations. Investigating tumor sub-populations of recurrent disease is important for adjusting therapy in recurrent EAC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12885-018-4789-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-12 /pmc/articles/PMC6134594/ /pubmed/30208867 http://dx.doi.org/10.1186/s12885-018-4789-4 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Case Report
Isidori, Federica
Malvi, Deborah
Fittipaldi, Silvia
Forcato, Claudio
Bozzarelli, Isotta
Sala, Claudia
Raulli, Giovanni
D’Errico, Antonia
Fiorentino, Michelangelo
Seri, Marco
Krishnadath, Kausilia K.
Bonora, Elena
Mattioli, Sandro
Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
title Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
title_full Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
title_fullStr Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
title_full_unstemmed Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
title_short Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
title_sort genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134594/
https://www.ncbi.nlm.nih.gov/pubmed/30208867
http://dx.doi.org/10.1186/s12885-018-4789-4
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