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Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)

Malignant tumors constitute a serious disease that threaten human life, and early diagnosis and metastasis prediction are critical to the choice of treatment plan and the timing of treatment. Integrin α(v)β(3), which has received broad attention as a molecular marker of the tumor neovasculature, is...

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Autores principales: Zhang, Yali, Zhu, Xuna, Liu, Lidong, Hong, Sen, Zuo, Zhichao, Wang, Peng, Su, Danke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134723/
https://www.ncbi.nlm.nih.gov/pubmed/30203331
http://dx.doi.org/10.1186/s11671-018-2695-y
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author Zhang, Yali
Zhu, Xuna
Liu, Lidong
Hong, Sen
Zuo, Zhichao
Wang, Peng
Su, Danke
author_facet Zhang, Yali
Zhu, Xuna
Liu, Lidong
Hong, Sen
Zuo, Zhichao
Wang, Peng
Su, Danke
author_sort Zhang, Yali
collection PubMed
description Malignant tumors constitute a serious disease that threaten human life, and early diagnosis and metastasis prediction are critical to the choice of treatment plan and the timing of treatment. Integrin α(v)β(3), which has received broad attention as a molecular marker of the tumor neovasculature, is an important target for monitoring tumorigenesis and progression in molecular imaging research. This study reports a magnetic resonance (MR)/fluorescence dual-mode molecular probe, cRGD-Gd-Cy5.5, which targets the integrin α(v)β(3) receptor and uses liposomes as carrier. The obtained nanoprobe had a size of 60.08 ± 0.45 nm, with good dispersion in water, a uniform distribution of sizes, desirable stability, and high relaxivity. Its r1 relaxation rate was 10.515 mM(−1) s(−1), much higher than that of other Gd chelates in clinical use. The probe showed no cytotoxicity at the tested concentrations in vitro, and its ability to target A549 cells and SUNE-1-5-8F cells was preliminarily evaluated through in vitro fluorescence imaging and MR imaging. The results demonstrated that the cRGD-Gd-Cy5.5 nanoprobe had good characteristics, showing desirable stability and biosafety, a high T1 relaxation rate, and strong targeting and binding to tumors with high expression of integrin α(v)β(3). Therefore, cRGD-Gd-Cy5.5 is a promising agent for the visual monitoring of tumor metastasis.
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spelling pubmed-61347232018-09-27 Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3) Zhang, Yali Zhu, Xuna Liu, Lidong Hong, Sen Zuo, Zhichao Wang, Peng Su, Danke Nanoscale Res Lett Nano Express Malignant tumors constitute a serious disease that threaten human life, and early diagnosis and metastasis prediction are critical to the choice of treatment plan and the timing of treatment. Integrin α(v)β(3), which has received broad attention as a molecular marker of the tumor neovasculature, is an important target for monitoring tumorigenesis and progression in molecular imaging research. This study reports a magnetic resonance (MR)/fluorescence dual-mode molecular probe, cRGD-Gd-Cy5.5, which targets the integrin α(v)β(3) receptor and uses liposomes as carrier. The obtained nanoprobe had a size of 60.08 ± 0.45 nm, with good dispersion in water, a uniform distribution of sizes, desirable stability, and high relaxivity. Its r1 relaxation rate was 10.515 mM(−1) s(−1), much higher than that of other Gd chelates in clinical use. The probe showed no cytotoxicity at the tested concentrations in vitro, and its ability to target A549 cells and SUNE-1-5-8F cells was preliminarily evaluated through in vitro fluorescence imaging and MR imaging. The results demonstrated that the cRGD-Gd-Cy5.5 nanoprobe had good characteristics, showing desirable stability and biosafety, a high T1 relaxation rate, and strong targeting and binding to tumors with high expression of integrin α(v)β(3). Therefore, cRGD-Gd-Cy5.5 is a promising agent for the visual monitoring of tumor metastasis. Springer US 2018-09-11 /pmc/articles/PMC6134723/ /pubmed/30203331 http://dx.doi.org/10.1186/s11671-018-2695-y Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Nano Express
Zhang, Yali
Zhu, Xuna
Liu, Lidong
Hong, Sen
Zuo, Zhichao
Wang, Peng
Su, Danke
Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)
title Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)
title_full Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)
title_fullStr Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)
title_full_unstemmed Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)
title_short Synthesis and In Vitro Study of a Dual-Mode Probe Targeting Integrin α(v)β(3)
title_sort synthesis and in vitro study of a dual-mode probe targeting integrin α(v)β(3)
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134723/
https://www.ncbi.nlm.nih.gov/pubmed/30203331
http://dx.doi.org/10.1186/s11671-018-2695-y
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