Sirt1 protects neural stem cells from apoptosis by decreasing acetylation of histone 3K9

OBJECTIVE: To explore the role and mechanism of Sirt1 in protecting neural stem cells (NSCs) from apoptosis. MATERIALS AND METHODS: Transfection was used to overexpress Sirt1 in rat NSCs. The effect of Sirt1 overexpression on camptothecin-induced apoptosis of NSCs was evaluated. Western blotting was...

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Detalles Bibliográficos
Autores principales: Jian, Chongdong, Zou, Cuihua, Xu, Ning, Chen, Guoying, Zou, Donghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135083/
https://www.ncbi.nlm.nih.gov/pubmed/30233218
http://dx.doi.org/10.2147/SCCAA.S173852
Descripción
Sumario:OBJECTIVE: To explore the role and mechanism of Sirt1 in protecting neural stem cells (NSCs) from apoptosis. MATERIALS AND METHODS: Transfection was used to overexpress Sirt1 in rat NSCs. The effect of Sirt1 overexpression on camptothecin-induced apoptosis of NSCs was evaluated. Western blotting was used to examine the expression of Sirt1, cleaved caspase-3, and acetylated histone 3K9. RESULTS: Overexpression of Sirt1 in NSCs decreased the cleavage of caspase-3 and acetylation of histone 3K9. CONCLUSION: Sirt1 may protect NSCs from apoptosis by decreasing the acetylation of histone 3 on K9.

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