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Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135222/ https://www.ncbi.nlm.nih.gov/pubmed/30122443 http://dx.doi.org/10.1016/j.stemcr.2018.07.007 |
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author | Veevers, Jennifer Farah, Elie N. Corselli, Mirko Witty, Alec D. Palomares, Karina Vidal, Jason G. Emre, Nil Carson, Christian T. Ouyang, Kunfu Liu, Canzhao van Vliet, Patrick Zhu, Maggie Hegarty, Jeffrey M. Deacon, Dekker C. Grinstein, Jonathan D. Dirschinger, Ralf J. Frazer, Kelly A. Adler, Eric D. Knowlton, Kirk U. Chi, Neil C. Martin, Jody C. Chen, Ju Evans, Sylvia M. |
author_facet | Veevers, Jennifer Farah, Elie N. Corselli, Mirko Witty, Alec D. Palomares, Karina Vidal, Jason G. Emre, Nil Carson, Christian T. Ouyang, Kunfu Liu, Canzhao van Vliet, Patrick Zhu, Maggie Hegarty, Jeffrey M. Deacon, Dekker C. Grinstein, Jonathan D. Dirschinger, Ralf J. Frazer, Kelly A. Adler, Eric D. Knowlton, Kirk U. Chi, Neil C. Martin, Jody C. Chen, Ju Evans, Sylvia M. |
author_sort | Veevers, Jennifer |
collection | PubMed |
description | To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial chromosome transgenic H9 hESC line in which GFP expression was driven by the human ventricular-specific myosin light chain 2 (MYL2) promoter was generated, and screened to identify cell-surface markers specific for MYL2-GFP-expressing VCMs. A CD77(+)/CD200(−) cell-surface signature facilitated isolation of >97% cardiac troponin I-positive cells from H9 hESC differentiation cultures, with 65% expressing MYL2-GFP. This study provides a tool for VCM enrichment when using some, but not all, human pluripotent stem cell lines. Tools generated in this study can be utilized toward understanding CM subtype specification, and enriching for VCMs for therapeutic applications. |
format | Online Article Text |
id | pubmed-6135222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61352222018-09-17 Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells Veevers, Jennifer Farah, Elie N. Corselli, Mirko Witty, Alec D. Palomares, Karina Vidal, Jason G. Emre, Nil Carson, Christian T. Ouyang, Kunfu Liu, Canzhao van Vliet, Patrick Zhu, Maggie Hegarty, Jeffrey M. Deacon, Dekker C. Grinstein, Jonathan D. Dirschinger, Ralf J. Frazer, Kelly A. Adler, Eric D. Knowlton, Kirk U. Chi, Neil C. Martin, Jody C. Chen, Ju Evans, Sylvia M. Stem Cell Reports Resource To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial chromosome transgenic H9 hESC line in which GFP expression was driven by the human ventricular-specific myosin light chain 2 (MYL2) promoter was generated, and screened to identify cell-surface markers specific for MYL2-GFP-expressing VCMs. A CD77(+)/CD200(−) cell-surface signature facilitated isolation of >97% cardiac troponin I-positive cells from H9 hESC differentiation cultures, with 65% expressing MYL2-GFP. This study provides a tool for VCM enrichment when using some, but not all, human pluripotent stem cell lines. Tools generated in this study can be utilized toward understanding CM subtype specification, and enriching for VCMs for therapeutic applications. Elsevier 2018-08-16 /pmc/articles/PMC6135222/ /pubmed/30122443 http://dx.doi.org/10.1016/j.stemcr.2018.07.007 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Resource Veevers, Jennifer Farah, Elie N. Corselli, Mirko Witty, Alec D. Palomares, Karina Vidal, Jason G. Emre, Nil Carson, Christian T. Ouyang, Kunfu Liu, Canzhao van Vliet, Patrick Zhu, Maggie Hegarty, Jeffrey M. Deacon, Dekker C. Grinstein, Jonathan D. Dirschinger, Ralf J. Frazer, Kelly A. Adler, Eric D. Knowlton, Kirk U. Chi, Neil C. Martin, Jody C. Chen, Ju Evans, Sylvia M. Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells |
title | Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells |
title_full | Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells |
title_fullStr | Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells |
title_full_unstemmed | Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells |
title_short | Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells |
title_sort | cell-surface marker signature for enrichment of ventricular cardiomyocytes derived from human embryonic stem cells |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135222/ https://www.ncbi.nlm.nih.gov/pubmed/30122443 http://dx.doi.org/10.1016/j.stemcr.2018.07.007 |
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