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Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells

To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial...

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Autores principales: Veevers, Jennifer, Farah, Elie N., Corselli, Mirko, Witty, Alec D., Palomares, Karina, Vidal, Jason G., Emre, Nil, Carson, Christian T., Ouyang, Kunfu, Liu, Canzhao, van Vliet, Patrick, Zhu, Maggie, Hegarty, Jeffrey M., Deacon, Dekker C., Grinstein, Jonathan D., Dirschinger, Ralf J., Frazer, Kelly A., Adler, Eric D., Knowlton, Kirk U., Chi, Neil C., Martin, Jody C., Chen, Ju, Evans, Sylvia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135222/
https://www.ncbi.nlm.nih.gov/pubmed/30122443
http://dx.doi.org/10.1016/j.stemcr.2018.07.007
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author Veevers, Jennifer
Farah, Elie N.
Corselli, Mirko
Witty, Alec D.
Palomares, Karina
Vidal, Jason G.
Emre, Nil
Carson, Christian T.
Ouyang, Kunfu
Liu, Canzhao
van Vliet, Patrick
Zhu, Maggie
Hegarty, Jeffrey M.
Deacon, Dekker C.
Grinstein, Jonathan D.
Dirschinger, Ralf J.
Frazer, Kelly A.
Adler, Eric D.
Knowlton, Kirk U.
Chi, Neil C.
Martin, Jody C.
Chen, Ju
Evans, Sylvia M.
author_facet Veevers, Jennifer
Farah, Elie N.
Corselli, Mirko
Witty, Alec D.
Palomares, Karina
Vidal, Jason G.
Emre, Nil
Carson, Christian T.
Ouyang, Kunfu
Liu, Canzhao
van Vliet, Patrick
Zhu, Maggie
Hegarty, Jeffrey M.
Deacon, Dekker C.
Grinstein, Jonathan D.
Dirschinger, Ralf J.
Frazer, Kelly A.
Adler, Eric D.
Knowlton, Kirk U.
Chi, Neil C.
Martin, Jody C.
Chen, Ju
Evans, Sylvia M.
author_sort Veevers, Jennifer
collection PubMed
description To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial chromosome transgenic H9 hESC line in which GFP expression was driven by the human ventricular-specific myosin light chain 2 (MYL2) promoter was generated, and screened to identify cell-surface markers specific for MYL2-GFP-expressing VCMs. A CD77(+)/CD200(−) cell-surface signature facilitated isolation of >97% cardiac troponin I-positive cells from H9 hESC differentiation cultures, with 65% expressing MYL2-GFP. This study provides a tool for VCM enrichment when using some, but not all, human pluripotent stem cell lines. Tools generated in this study can be utilized toward understanding CM subtype specification, and enriching for VCMs for therapeutic applications.
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spelling pubmed-61352222018-09-17 Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells Veevers, Jennifer Farah, Elie N. Corselli, Mirko Witty, Alec D. Palomares, Karina Vidal, Jason G. Emre, Nil Carson, Christian T. Ouyang, Kunfu Liu, Canzhao van Vliet, Patrick Zhu, Maggie Hegarty, Jeffrey M. Deacon, Dekker C. Grinstein, Jonathan D. Dirschinger, Ralf J. Frazer, Kelly A. Adler, Eric D. Knowlton, Kirk U. Chi, Neil C. Martin, Jody C. Chen, Ju Evans, Sylvia M. Stem Cell Reports Resource To facilitate understanding of human cardiomyocyte (CM) subtype specification, and the study of ventricular CM biology in particular, we developed a broadly applicable strategy for enrichment of ventricular cardiomyocytes (VCMs) derived from human embryonic stem cells (hESCs). A bacterial artificial chromosome transgenic H9 hESC line in which GFP expression was driven by the human ventricular-specific myosin light chain 2 (MYL2) promoter was generated, and screened to identify cell-surface markers specific for MYL2-GFP-expressing VCMs. A CD77(+)/CD200(−) cell-surface signature facilitated isolation of >97% cardiac troponin I-positive cells from H9 hESC differentiation cultures, with 65% expressing MYL2-GFP. This study provides a tool for VCM enrichment when using some, but not all, human pluripotent stem cell lines. Tools generated in this study can be utilized toward understanding CM subtype specification, and enriching for VCMs for therapeutic applications. Elsevier 2018-08-16 /pmc/articles/PMC6135222/ /pubmed/30122443 http://dx.doi.org/10.1016/j.stemcr.2018.07.007 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Resource
Veevers, Jennifer
Farah, Elie N.
Corselli, Mirko
Witty, Alec D.
Palomares, Karina
Vidal, Jason G.
Emre, Nil
Carson, Christian T.
Ouyang, Kunfu
Liu, Canzhao
van Vliet, Patrick
Zhu, Maggie
Hegarty, Jeffrey M.
Deacon, Dekker C.
Grinstein, Jonathan D.
Dirschinger, Ralf J.
Frazer, Kelly A.
Adler, Eric D.
Knowlton, Kirk U.
Chi, Neil C.
Martin, Jody C.
Chen, Ju
Evans, Sylvia M.
Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
title Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
title_full Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
title_fullStr Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
title_full_unstemmed Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
title_short Cell-Surface Marker Signature for Enrichment of Ventricular Cardiomyocytes Derived from Human Embryonic Stem Cells
title_sort cell-surface marker signature for enrichment of ventricular cardiomyocytes derived from human embryonic stem cells
topic Resource
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135222/
https://www.ncbi.nlm.nih.gov/pubmed/30122443
http://dx.doi.org/10.1016/j.stemcr.2018.07.007
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