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Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality

IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1-deficient m...

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Autores principales: Orlacchio, Arturo, Stark, Aaron E., Foray, Claudia, Amari, Foued, Sheetz, Tyler, Reese, Erika, Tessari, Anna, La Perle, Krista, Palmieri, Dario, Tsichlis, Philip N., Coppola, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135376/
https://www.ncbi.nlm.nih.gov/pubmed/30208029
http://dx.doi.org/10.1371/journal.pone.0201030
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author Orlacchio, Arturo
Stark, Aaron E.
Foray, Claudia
Amari, Foued
Sheetz, Tyler
Reese, Erika
Tessari, Anna
La Perle, Krista
Palmieri, Dario
Tsichlis, Philip N.
Coppola, Vincenzo
author_facet Orlacchio, Arturo
Stark, Aaron E.
Foray, Claudia
Amari, Foued
Sheetz, Tyler
Reese, Erika
Tessari, Anna
La Perle, Krista
Palmieri, Dario
Tsichlis, Philip N.
Coppola, Vincenzo
author_sort Orlacchio, Arturo
collection PubMed
description IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1-deficient mice. A thorough immunohistochemical characterization shows that IWS1 protein is present in the nucleus of all cells in most of the examined tissues, with few notable exceptions. We also report that ablation of Iws1 consistently causes lethality at the pre-implantation stage with high expression of the gene in fertilized oocytes. In summary, we are providing evidence that Iws1 is expressed in all adult organs and it is an essential gene for mouse embryonic development.
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spelling pubmed-61353762018-09-27 Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality Orlacchio, Arturo Stark, Aaron E. Foray, Claudia Amari, Foued Sheetz, Tyler Reese, Erika Tessari, Anna La Perle, Krista Palmieri, Dario Tsichlis, Philip N. Coppola, Vincenzo PLoS One Research Article IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1-deficient mice. A thorough immunohistochemical characterization shows that IWS1 protein is present in the nucleus of all cells in most of the examined tissues, with few notable exceptions. We also report that ablation of Iws1 consistently causes lethality at the pre-implantation stage with high expression of the gene in fertilized oocytes. In summary, we are providing evidence that Iws1 is expressed in all adult organs and it is an essential gene for mouse embryonic development. Public Library of Science 2018-09-12 /pmc/articles/PMC6135376/ /pubmed/30208029 http://dx.doi.org/10.1371/journal.pone.0201030 Text en © 2018 Orlacchio et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Orlacchio, Arturo
Stark, Aaron E.
Foray, Claudia
Amari, Foued
Sheetz, Tyler
Reese, Erika
Tessari, Anna
La Perle, Krista
Palmieri, Dario
Tsichlis, Philip N.
Coppola, Vincenzo
Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
title Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
title_full Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
title_fullStr Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
title_full_unstemmed Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
title_short Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
title_sort genetic ablation of interacting with spt6 (iws1) causes early embryonic lethality
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135376/
https://www.ncbi.nlm.nih.gov/pubmed/30208029
http://dx.doi.org/10.1371/journal.pone.0201030
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