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Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality
IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1-deficient m...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135376/ https://www.ncbi.nlm.nih.gov/pubmed/30208029 http://dx.doi.org/10.1371/journal.pone.0201030 |
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author | Orlacchio, Arturo Stark, Aaron E. Foray, Claudia Amari, Foued Sheetz, Tyler Reese, Erika Tessari, Anna La Perle, Krista Palmieri, Dario Tsichlis, Philip N. Coppola, Vincenzo |
author_facet | Orlacchio, Arturo Stark, Aaron E. Foray, Claudia Amari, Foued Sheetz, Tyler Reese, Erika Tessari, Anna La Perle, Krista Palmieri, Dario Tsichlis, Philip N. Coppola, Vincenzo |
author_sort | Orlacchio, Arturo |
collection | PubMed |
description | IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1-deficient mice. A thorough immunohistochemical characterization shows that IWS1 protein is present in the nucleus of all cells in most of the examined tissues, with few notable exceptions. We also report that ablation of Iws1 consistently causes lethality at the pre-implantation stage with high expression of the gene in fertilized oocytes. In summary, we are providing evidence that Iws1 is expressed in all adult organs and it is an essential gene for mouse embryonic development. |
format | Online Article Text |
id | pubmed-6135376 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-61353762018-09-27 Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality Orlacchio, Arturo Stark, Aaron E. Foray, Claudia Amari, Foued Sheetz, Tyler Reese, Erika Tessari, Anna La Perle, Krista Palmieri, Dario Tsichlis, Philip N. Coppola, Vincenzo PLoS One Research Article IWS1 is an RNA-polymerase II (RNAPII)-associated transcription elongation factor whose biological functions are poorly characterized. To shed some light on the function of this protein at the organismal level, we performed a systematic tissue analysis of its expression and generated Iws1-deficient mice. A thorough immunohistochemical characterization shows that IWS1 protein is present in the nucleus of all cells in most of the examined tissues, with few notable exceptions. We also report that ablation of Iws1 consistently causes lethality at the pre-implantation stage with high expression of the gene in fertilized oocytes. In summary, we are providing evidence that Iws1 is expressed in all adult organs and it is an essential gene for mouse embryonic development. Public Library of Science 2018-09-12 /pmc/articles/PMC6135376/ /pubmed/30208029 http://dx.doi.org/10.1371/journal.pone.0201030 Text en © 2018 Orlacchio et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Orlacchio, Arturo Stark, Aaron E. Foray, Claudia Amari, Foued Sheetz, Tyler Reese, Erika Tessari, Anna La Perle, Krista Palmieri, Dario Tsichlis, Philip N. Coppola, Vincenzo Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality |
title | Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality |
title_full | Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality |
title_fullStr | Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality |
title_full_unstemmed | Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality |
title_short | Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality |
title_sort | genetic ablation of interacting with spt6 (iws1) causes early embryonic lethality |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135376/ https://www.ncbi.nlm.nih.gov/pubmed/30208029 http://dx.doi.org/10.1371/journal.pone.0201030 |
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