Cargando…

The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice

This experiment was conducted to investigate the effect of exposure to high altitude and low oxygen on intestinal microbial communities using mice as an animal model. Fecal microbiota from mice housed in a control environment representing 2,200 meters (NC group) above sea level with 16% Oxygen and m...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Wei, Jiao, Lefei, Liu, Ruixin, Zhang, Yu, Ji, Qiaorong, Zhang, Huan, Gao, Xiang, Ma, Yan, Shi, Hai Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135514/
https://www.ncbi.nlm.nih.gov/pubmed/30208088
http://dx.doi.org/10.1371/journal.pone.0203701
_version_ 1783354840932417536
author Zhang, Wei
Jiao, Lefei
Liu, Ruixin
Zhang, Yu
Ji, Qiaorong
Zhang, Huan
Gao, Xiang
Ma, Yan
Shi, Hai Ning
author_facet Zhang, Wei
Jiao, Lefei
Liu, Ruixin
Zhang, Yu
Ji, Qiaorong
Zhang, Huan
Gao, Xiang
Ma, Yan
Shi, Hai Ning
author_sort Zhang, Wei
collection PubMed
description This experiment was conducted to investigate the effect of exposure to high altitude and low oxygen on intestinal microbial communities using mice as an animal model. Fecal microbiota from mice housed in a control environment representing 2,200 meters (NC group) above sea level with 16% Oxygen and mice that were placed in a hypobaric chamber representing 5000 meters (HC group) above sea level with 11% Oxygen for 30 days, were analyzed by the HiSeq Illumina sequencing platform. The results showed a significant difference in beta diversity observed between the two groups, while no significant difference was observed in alpha diversity. Compared with the NC group, the relative abundance of class Epsilonproteobacteria, phlym Actinobacteria, class Erysipelotrichia and genus Helicobacter were significantly lower (P<0.05), while the relative abundance of genus Alistipes was increased in the HC group; Phenotypic analysis showed no significant difference in aerobic, anaerobic, facultatively anaerobic, potentially pathogenic, stress tolerant, mobile element, biofilms formation, gram negative and gram positive between HC group and NC group; Functional analysis results showed significant differences in 34 gene functional metabolic pathways (carbohydrate digestion and absorption, energy metabolism, arachidonic acid metabolism, flavonoid biosynthesis, RIG-I-like receptor signaling pathway, etc) between HC group and NC group. Together, these findings suggest that exposure to high altitude and low oxygen had the potential to change the intestinal microbial communities, which potentially may modulate metabolic processes in mice.
format Online
Article
Text
id pubmed-6135514
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-61355142018-09-27 The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice Zhang, Wei Jiao, Lefei Liu, Ruixin Zhang, Yu Ji, Qiaorong Zhang, Huan Gao, Xiang Ma, Yan Shi, Hai Ning PLoS One Research Article This experiment was conducted to investigate the effect of exposure to high altitude and low oxygen on intestinal microbial communities using mice as an animal model. Fecal microbiota from mice housed in a control environment representing 2,200 meters (NC group) above sea level with 16% Oxygen and mice that were placed in a hypobaric chamber representing 5000 meters (HC group) above sea level with 11% Oxygen for 30 days, were analyzed by the HiSeq Illumina sequencing platform. The results showed a significant difference in beta diversity observed between the two groups, while no significant difference was observed in alpha diversity. Compared with the NC group, the relative abundance of class Epsilonproteobacteria, phlym Actinobacteria, class Erysipelotrichia and genus Helicobacter were significantly lower (P<0.05), while the relative abundance of genus Alistipes was increased in the HC group; Phenotypic analysis showed no significant difference in aerobic, anaerobic, facultatively anaerobic, potentially pathogenic, stress tolerant, mobile element, biofilms formation, gram negative and gram positive between HC group and NC group; Functional analysis results showed significant differences in 34 gene functional metabolic pathways (carbohydrate digestion and absorption, energy metabolism, arachidonic acid metabolism, flavonoid biosynthesis, RIG-I-like receptor signaling pathway, etc) between HC group and NC group. Together, these findings suggest that exposure to high altitude and low oxygen had the potential to change the intestinal microbial communities, which potentially may modulate metabolic processes in mice. Public Library of Science 2018-09-12 /pmc/articles/PMC6135514/ /pubmed/30208088 http://dx.doi.org/10.1371/journal.pone.0203701 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Wei
Jiao, Lefei
Liu, Ruixin
Zhang, Yu
Ji, Qiaorong
Zhang, Huan
Gao, Xiang
Ma, Yan
Shi, Hai Ning
The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
title The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
title_full The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
title_fullStr The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
title_full_unstemmed The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
title_short The effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
title_sort effect of exposure to high altitude and low oxygen on intestinal microbial communities in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135514/
https://www.ncbi.nlm.nih.gov/pubmed/30208088
http://dx.doi.org/10.1371/journal.pone.0203701
work_keys_str_mv AT zhangwei theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT jiaolefei theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT liuruixin theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT zhangyu theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT jiqiaorong theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT zhanghuan theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT gaoxiang theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT mayan theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT shihaining theeffectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT zhangwei effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT jiaolefei effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT liuruixin effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT zhangyu effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT jiqiaorong effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT zhanghuan effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT gaoxiang effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT mayan effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice
AT shihaining effectofexposuretohighaltitudeandlowoxygenonintestinalmicrobialcommunitiesinmice