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Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system
Spiral ganglion (SG) neurons of the cochlea convey all auditory inputs to the brain, yet the cellular and molecular complexity necessary to decode the various acoustic features in the SG has remained unresolved. Using single-cell RNA sequencing, we identify four types of SG neurons, including three...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135759/ https://www.ncbi.nlm.nih.gov/pubmed/30209249 http://dx.doi.org/10.1038/s41467-018-06033-3 |
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author | Petitpré, Charles Wu, Haohao Sharma, Anil Tokarska, Anna Fontanet, Paula Wang, Yiqiao Helmbacher, Françoise Yackle, Kevin Silberberg, Gilad Hadjab, Saida Lallemend, François |
author_facet | Petitpré, Charles Wu, Haohao Sharma, Anil Tokarska, Anna Fontanet, Paula Wang, Yiqiao Helmbacher, Françoise Yackle, Kevin Silberberg, Gilad Hadjab, Saida Lallemend, François |
author_sort | Petitpré, Charles |
collection | PubMed |
description | Spiral ganglion (SG) neurons of the cochlea convey all auditory inputs to the brain, yet the cellular and molecular complexity necessary to decode the various acoustic features in the SG has remained unresolved. Using single-cell RNA sequencing, we identify four types of SG neurons, including three novel subclasses of type I neurons and the type II neurons, and provide a comprehensive genetic framework that define their potential synaptic communication patterns. The connectivity patterns of the three subclasses of type I neurons with inner hair cells and their electrophysiological profiles suggest that they represent the intensity-coding properties of auditory afferents. Moreover, neuron type specification is already established at birth, indicating a neuronal diversification process independent of neuronal activity. Thus, this work provides a transcriptional catalog of neuron types in the cochlea, which serves as a valuable resource for dissecting cell-type-specific functions of dedicated afferents in auditory perception and in hearing disorders. |
format | Online Article Text |
id | pubmed-6135759 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61357592018-09-14 Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system Petitpré, Charles Wu, Haohao Sharma, Anil Tokarska, Anna Fontanet, Paula Wang, Yiqiao Helmbacher, Françoise Yackle, Kevin Silberberg, Gilad Hadjab, Saida Lallemend, François Nat Commun Article Spiral ganglion (SG) neurons of the cochlea convey all auditory inputs to the brain, yet the cellular and molecular complexity necessary to decode the various acoustic features in the SG has remained unresolved. Using single-cell RNA sequencing, we identify four types of SG neurons, including three novel subclasses of type I neurons and the type II neurons, and provide a comprehensive genetic framework that define their potential synaptic communication patterns. The connectivity patterns of the three subclasses of type I neurons with inner hair cells and their electrophysiological profiles suggest that they represent the intensity-coding properties of auditory afferents. Moreover, neuron type specification is already established at birth, indicating a neuronal diversification process independent of neuronal activity. Thus, this work provides a transcriptional catalog of neuron types in the cochlea, which serves as a valuable resource for dissecting cell-type-specific functions of dedicated afferents in auditory perception and in hearing disorders. Nature Publishing Group UK 2018-09-12 /pmc/articles/PMC6135759/ /pubmed/30209249 http://dx.doi.org/10.1038/s41467-018-06033-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Petitpré, Charles Wu, Haohao Sharma, Anil Tokarska, Anna Fontanet, Paula Wang, Yiqiao Helmbacher, Françoise Yackle, Kevin Silberberg, Gilad Hadjab, Saida Lallemend, François Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
title | Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
title_full | Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
title_fullStr | Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
title_full_unstemmed | Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
title_short | Neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
title_sort | neuronal heterogeneity and stereotyped connectivity in the auditory afferent system |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6135759/ https://www.ncbi.nlm.nih.gov/pubmed/30209249 http://dx.doi.org/10.1038/s41467-018-06033-3 |
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