Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

CRISPR/Cas9 is a programmable nuclease composed of the Cas9 protein and a guide RNA (gRNA) molecule. To create a mutant potato, a powerful genome-editing system was required because potato has a tetraploid genome. The translational enhancer dMac3, consisting of a portion of the OsMac3 mRNA 5′-untran...

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Detalles Bibliográficos
Autores principales: Kusano, Hiroaki, Ohnuma, Mariko, Mutsuro-Aoki, Hiromi, Asahi, Takahiro, Ichinosawa, Dai, Onodera, Hitomi, Asano, Kenji, Noda, Takahiro, Horie, Takaaki, Fukumoto, Kou, Kihira, Miho, Teramura, Hiroshi, Yazaki, Kazufumi, Umemoto, Naoyuki, Muranaka, Toshiya, Shimada, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137036/
https://www.ncbi.nlm.nih.gov/pubmed/30214055
http://dx.doi.org/10.1038/s41598-018-32049-2

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