Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst

Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-a...

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Autores principales: Lekmeechai, Sujinna, Su, Yu-Ching, Brant, Marta, Alvarado-Kristensson, Maria, Vallström, Anna, Obi, Ikenna, Arnqvist, Anna, Riesbeck, Kristian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137165/
https://www.ncbi.nlm.nih.gov/pubmed/30245670
http://dx.doi.org/10.3389/fmicb.2018.01837
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author Lekmeechai, Sujinna
Su, Yu-Ching
Brant, Marta
Alvarado-Kristensson, Maria
Vallström, Anna
Obi, Ikenna
Arnqvist, Anna
Riesbeck, Kristian
author_facet Lekmeechai, Sujinna
Su, Yu-Ching
Brant, Marta
Alvarado-Kristensson, Maria
Vallström, Anna
Obi, Ikenna
Arnqvist, Anna
Riesbeck, Kristian
author_sort Lekmeechai, Sujinna
collection PubMed
description Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H(2)O(2) and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.
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spelling pubmed-61371652018-09-21 Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst Lekmeechai, Sujinna Su, Yu-Ching Brant, Marta Alvarado-Kristensson, Maria Vallström, Anna Obi, Ikenna Arnqvist, Anna Riesbeck, Kristian Front Microbiol Microbiology Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H(2)O(2) and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion. Frontiers Media S.A. 2018-09-07 /pmc/articles/PMC6137165/ /pubmed/30245670 http://dx.doi.org/10.3389/fmicb.2018.01837 Text en Copyright © 2018 Lekmeechai, Su, Brant, Alvarado-Kristensson, Vallström, Obi, Arnqvist and Riesbeck. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lekmeechai, Sujinna
Su, Yu-Ching
Brant, Marta
Alvarado-Kristensson, Maria
Vallström, Anna
Obi, Ikenna
Arnqvist, Anna
Riesbeck, Kristian
Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst
title Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst
title_full Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst
title_fullStr Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst
title_full_unstemmed Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst
title_short Helicobacter pylori Outer Membrane Vesicles Protect the Pathogen From Reactive Oxygen Species of the Respiratory Burst
title_sort helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137165/
https://www.ncbi.nlm.nih.gov/pubmed/30245670
http://dx.doi.org/10.3389/fmicb.2018.01837
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