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A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer

Lung cancer is a malignant tumor with high fatality rate and causes great harm to human economic life. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. With the rapid development of epigenetic study in the last decade, the understanding of the pathogenesis of lung cancer an...

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Autores principales: Chen, Bifeng, Wang, Jingdong, Wang, Jieling, Wang, Huan, Gu, Xiuli, Tang, Liang, Feng, Xianhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137244/
https://www.ncbi.nlm.nih.gov/pubmed/30054425
http://dx.doi.org/10.1042/BSR20180678
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author Chen, Bifeng
Wang, Jingdong
Wang, Jieling
Wang, Huan
Gu, Xiuli
Tang, Liang
Feng, Xianhong
author_facet Chen, Bifeng
Wang, Jingdong
Wang, Jieling
Wang, Huan
Gu, Xiuli
Tang, Liang
Feng, Xianhong
author_sort Chen, Bifeng
collection PubMed
description Lung cancer is a malignant tumor with high fatality rate and causes great harm to human economic life. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. With the rapid development of epigenetic study in the last decade, the understanding of the pathogenesis of lung cancer and the development of personalized treatment of lung cancer are picking up pace. Previous studies showed that miR-29 family members (miR-29s; miR-29a, -29b, and -29c) are down-regulated in most human cancers, including NSCLC, but their biological roles in tumorigenesis and their regulation mechanism are still not fully elucidated. Herein, we reported that the miR-29a, -29b and, -29c were coincidently down-regulated in NSCLC, and the histone H3K9 methyltransferase SET domain, bifurcated 1 (SETDB1) was directly targetted by miR-29s. Moreover, SETDB1 negatively regulated the expression of TP53 and overexpression of SETDB1 down-regulating the expression of miR-29s, while TP53 positively regulated the expression of miR-29s and overexpression of TP53 down-regulated the expression of SETDB1. On the other side, as a downstream target of TP53, the H3K9 methyltransferase Suv39h1 was also down-regulated by miR-29s via up-regulating TP53 expression. The further detection of H3K9 methylation status after changes in miR-29s expression revealed that they negatively regulated the levels of H3K9 di- and trimethylation in NSCLC. Collectively, our findings highlight a TP53/miR-29s/SETDB1 regulatory circuitry and assign a role of H3K9 methylation regulator to miR-29s, which may be a potential therapeutic target in the treatment of NSCLC.
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spelling pubmed-61372442018-09-18 A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer Chen, Bifeng Wang, Jingdong Wang, Jieling Wang, Huan Gu, Xiuli Tang, Liang Feng, Xianhong Biosci Rep Research Articles Lung cancer is a malignant tumor with high fatality rate and causes great harm to human economic life. Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. With the rapid development of epigenetic study in the last decade, the understanding of the pathogenesis of lung cancer and the development of personalized treatment of lung cancer are picking up pace. Previous studies showed that miR-29 family members (miR-29s; miR-29a, -29b, and -29c) are down-regulated in most human cancers, including NSCLC, but their biological roles in tumorigenesis and their regulation mechanism are still not fully elucidated. Herein, we reported that the miR-29a, -29b and, -29c were coincidently down-regulated in NSCLC, and the histone H3K9 methyltransferase SET domain, bifurcated 1 (SETDB1) was directly targetted by miR-29s. Moreover, SETDB1 negatively regulated the expression of TP53 and overexpression of SETDB1 down-regulating the expression of miR-29s, while TP53 positively regulated the expression of miR-29s and overexpression of TP53 down-regulated the expression of SETDB1. On the other side, as a downstream target of TP53, the H3K9 methyltransferase Suv39h1 was also down-regulated by miR-29s via up-regulating TP53 expression. The further detection of H3K9 methylation status after changes in miR-29s expression revealed that they negatively regulated the levels of H3K9 di- and trimethylation in NSCLC. Collectively, our findings highlight a TP53/miR-29s/SETDB1 regulatory circuitry and assign a role of H3K9 methylation regulator to miR-29s, which may be a potential therapeutic target in the treatment of NSCLC. Portland Press Ltd. 2018-09-14 /pmc/articles/PMC6137244/ /pubmed/30054425 http://dx.doi.org/10.1042/BSR20180678 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Chen, Bifeng
Wang, Jingdong
Wang, Jieling
Wang, Huan
Gu, Xiuli
Tang, Liang
Feng, Xianhong
A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
title A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
title_full A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
title_fullStr A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
title_full_unstemmed A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
title_short A regulatory circuitry comprising TP53, miR-29 family, and SETDB1 in non-small cell lung cancer
title_sort regulatory circuitry comprising tp53, mir-29 family, and setdb1 in non-small cell lung cancer
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137244/
https://www.ncbi.nlm.nih.gov/pubmed/30054425
http://dx.doi.org/10.1042/BSR20180678
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