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Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae

Haemonchus contortus is one of the prevalent and pathogenic ruminant parasites that has grown resistance to common anthelmintic treatment. This study evaluated the anthelmintic potential of Moringa oleifera seed ethanolic and aqueous extracts against H. contortus eggs and infective stage larvae (L3s...

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Autores principales: Cabardo, Delfin E., Portugaliza, Harvie P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine, Cairo University 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137834/
https://www.ncbi.nlm.nih.gov/pubmed/30255045
http://dx.doi.org/10.1016/j.ijvsm.2017.02.001
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author Cabardo, Delfin E.
Portugaliza, Harvie P.
author_facet Cabardo, Delfin E.
Portugaliza, Harvie P.
author_sort Cabardo, Delfin E.
collection PubMed
description Haemonchus contortus is one of the prevalent and pathogenic ruminant parasites that has grown resistance to common anthelmintic treatment. This study evaluated the anthelmintic potential of Moringa oleifera seed ethanolic and aqueous extracts against H. contortus eggs and infective stage larvae (L3s). The efficacy of five extract concentrations (0.95, 1.95, 3.9, 7.8, and 15.6 mg/mL) were tested through egg hatch assay and larval motility test. Phytochemical tests were conducted to detect the different plant secondary metabolites in the extracts. In the ovicidal assay, the ethanolic and aqueous extracts showed 95.89% and 81.72% egg hatch inhibition at 15.6 mg/mL, respectively. The ovicidal activity of 15.6 mg/mL ethanolic extract was comparable with that of albendazole (p > 0.05). The LC(50) against the eggs was recorded at 2.91 and 3.83 mg/mL for ethanolic and aqueous extracts, respectively. In the larvicidal assay, the ethanolic and aqueous extracts exhibited 56.94% and 92.50% efficacy at 7.8 mg/mL, respectively. The larvicidal activity of 7.8 mg/mL aqueous extract was similar statistically with that of ivermectin (p > 0.05). The LC(50) against L3s was recorded at 6.96 and 4.12 mg/mL for ethanolic and aqueous extracts, respectively. The secondary metabolites detected were tannins in ethanolic extract and saponins in aqueous extract. Both extracts inhibited larvae formation inside the eggs and rendered the L3s immobile. Therefore, M. oleifera seed extracts contained plant bioactive compounds with anthelmintic property against the eggs and L3s of H. contortus.
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spelling pubmed-61378342018-09-25 Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae Cabardo, Delfin E. Portugaliza, Harvie P. Int J Vet Sci Med Original Research Article Haemonchus contortus is one of the prevalent and pathogenic ruminant parasites that has grown resistance to common anthelmintic treatment. This study evaluated the anthelmintic potential of Moringa oleifera seed ethanolic and aqueous extracts against H. contortus eggs and infective stage larvae (L3s). The efficacy of five extract concentrations (0.95, 1.95, 3.9, 7.8, and 15.6 mg/mL) were tested through egg hatch assay and larval motility test. Phytochemical tests were conducted to detect the different plant secondary metabolites in the extracts. In the ovicidal assay, the ethanolic and aqueous extracts showed 95.89% and 81.72% egg hatch inhibition at 15.6 mg/mL, respectively. The ovicidal activity of 15.6 mg/mL ethanolic extract was comparable with that of albendazole (p > 0.05). The LC(50) against the eggs was recorded at 2.91 and 3.83 mg/mL for ethanolic and aqueous extracts, respectively. In the larvicidal assay, the ethanolic and aqueous extracts exhibited 56.94% and 92.50% efficacy at 7.8 mg/mL, respectively. The larvicidal activity of 7.8 mg/mL aqueous extract was similar statistically with that of ivermectin (p > 0.05). The LC(50) against L3s was recorded at 6.96 and 4.12 mg/mL for ethanolic and aqueous extracts, respectively. The secondary metabolites detected were tannins in ethanolic extract and saponins in aqueous extract. Both extracts inhibited larvae formation inside the eggs and rendered the L3s immobile. Therefore, M. oleifera seed extracts contained plant bioactive compounds with anthelmintic property against the eggs and L3s of H. contortus. Faculty of Veterinary Medicine, Cairo University 2017-04-12 /pmc/articles/PMC6137834/ /pubmed/30255045 http://dx.doi.org/10.1016/j.ijvsm.2017.02.001 Text en © 2017 Faculty of Veterinary Medicine, Cairo University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research Article
Cabardo, Delfin E.
Portugaliza, Harvie P.
Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae
title Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae
title_full Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae
title_fullStr Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae
title_full_unstemmed Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae
title_short Anthelmintic activity of Moringa oleifera seed aqueous and ethanolic extracts against Haemonchus contortus eggs and third stage larvae
title_sort anthelmintic activity of moringa oleifera seed aqueous and ethanolic extracts against haemonchus contortus eggs and third stage larvae
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137834/
https://www.ncbi.nlm.nih.gov/pubmed/30255045
http://dx.doi.org/10.1016/j.ijvsm.2017.02.001
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