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Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming
Genome architecture has emerged as a critical element of transcriptional regulation, although its role in the control of cell identity is not well understood. Here we use transcription factor (TF)-mediated reprogramming to examine the interplay between genome architecture and transcriptional program...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137960/ https://www.ncbi.nlm.nih.gov/pubmed/30240614 http://dx.doi.org/10.1016/j.isci.2018.08.002 |
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author | Liu, Sijia Chen, Haiming Ronquist, Scott Seaman, Laura Ceglia, Nicholas Meixner, Walter Chen, Pin-Yu Higgins, Gerald Baldi, Pierre Smale, Steve Hero, Alfred Muir, Lindsey A. Rajapakse, Indika |
author_facet | Liu, Sijia Chen, Haiming Ronquist, Scott Seaman, Laura Ceglia, Nicholas Meixner, Walter Chen, Pin-Yu Higgins, Gerald Baldi, Pierre Smale, Steve Hero, Alfred Muir, Lindsey A. Rajapakse, Indika |
author_sort | Liu, Sijia |
collection | PubMed |
description | Genome architecture has emerged as a critical element of transcriptional regulation, although its role in the control of cell identity is not well understood. Here we use transcription factor (TF)-mediated reprogramming to examine the interplay between genome architecture and transcriptional programs that transition cells into the myogenic identity. We recently developed new methods for evaluating the topological features of genome architecture based on network centrality. Through integrated analysis of these features of genome architecture and transcriptome dynamics during myogenic reprogramming of human fibroblasts we find that significant architectural reorganization precedes activation of a myogenic transcriptional program. This interplay sets the stage for a critical transition observed at several genomic scales reflecting definitive adoption of the myogenic phenotype. Subsequently, TFs within the myogenic transcriptional program participate in entrainment of biological rhythms. These findings reveal a role for topological features of genome architecture in the initiation of transcriptional programs during TF-mediated human cellular reprogramming. |
format | Online Article Text |
id | pubmed-6137960 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-61379602018-09-17 Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming Liu, Sijia Chen, Haiming Ronquist, Scott Seaman, Laura Ceglia, Nicholas Meixner, Walter Chen, Pin-Yu Higgins, Gerald Baldi, Pierre Smale, Steve Hero, Alfred Muir, Lindsey A. Rajapakse, Indika iScience Article Genome architecture has emerged as a critical element of transcriptional regulation, although its role in the control of cell identity is not well understood. Here we use transcription factor (TF)-mediated reprogramming to examine the interplay between genome architecture and transcriptional programs that transition cells into the myogenic identity. We recently developed new methods for evaluating the topological features of genome architecture based on network centrality. Through integrated analysis of these features of genome architecture and transcriptome dynamics during myogenic reprogramming of human fibroblasts we find that significant architectural reorganization precedes activation of a myogenic transcriptional program. This interplay sets the stage for a critical transition observed at several genomic scales reflecting definitive adoption of the myogenic phenotype. Subsequently, TFs within the myogenic transcriptional program participate in entrainment of biological rhythms. These findings reveal a role for topological features of genome architecture in the initiation of transcriptional programs during TF-mediated human cellular reprogramming. Elsevier 2018-08-08 /pmc/articles/PMC6137960/ /pubmed/30240614 http://dx.doi.org/10.1016/j.isci.2018.08.002 Text en © 2018. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Liu, Sijia Chen, Haiming Ronquist, Scott Seaman, Laura Ceglia, Nicholas Meixner, Walter Chen, Pin-Yu Higgins, Gerald Baldi, Pierre Smale, Steve Hero, Alfred Muir, Lindsey A. Rajapakse, Indika Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming |
title | Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming |
title_full | Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming |
title_fullStr | Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming |
title_full_unstemmed | Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming |
title_short | Genome Architecture Mediates Transcriptional Control of Human Myogenic Reprogramming |
title_sort | genome architecture mediates transcriptional control of human myogenic reprogramming |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137960/ https://www.ncbi.nlm.nih.gov/pubmed/30240614 http://dx.doi.org/10.1016/j.isci.2018.08.002 |
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