One‐step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock‐in mice

Here, we describe a one‐step, in vivo CRISPR/Cas9 nuclease‐mediated strategy to generate knock‐in mice. We produced knock‐in (KI) mice wherein a 1.9‐kb DNA fragment bearing a pre‐arranged human B‐cell receptor heavy chain was recombined into the native murine immunoglobulin locus. Our methodology re...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Ying‐Cing, Pecetta, Simone, Steichen, Jon M, Kratochvil, Sven, Melzi, Eleonora, Arnold, Johan, Dougan, Stephanie K, Wu, Lin, Kirsch, Kathrin H, Nair, Usha, Schief, William R, Batista, Facundo D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138433/
https://www.ncbi.nlm.nih.gov/pubmed/30087111
http://dx.doi.org/10.15252/embj.201899243

Ejemplares similares

Ejemplares similares