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The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva

Selenium (Se) might be protective against oxidative stress at nutritional levels, but elevated Se concentrations in the diet has been revealed as the main culprit for the extinction of natural fish populations in Se-contaminated lakes. Though Se predominate as waterborne selenite (IV) and selenate (...

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Autores principales: Ma, Shanshan, Zeng, Xiangfeng, Chen, Hongxing, Geng, Shicong, Yan, Liang, Luo, Yongju, Xie, Lingtian, Zhang, Qianru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138650/
https://www.ncbi.nlm.nih.gov/pubmed/30218092
http://dx.doi.org/10.1038/s41598-018-32270-z
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author Ma, Shanshan
Zeng, Xiangfeng
Chen, Hongxing
Geng, Shicong
Yan, Liang
Luo, Yongju
Xie, Lingtian
Zhang, Qianru
author_facet Ma, Shanshan
Zeng, Xiangfeng
Chen, Hongxing
Geng, Shicong
Yan, Liang
Luo, Yongju
Xie, Lingtian
Zhang, Qianru
author_sort Ma, Shanshan
collection PubMed
description Selenium (Se) might be protective against oxidative stress at nutritional levels, but elevated Se concentrations in the diet has been revealed as the main culprit for the extinction of natural fish populations in Se-contaminated lakes. Though Se predominate as waterborne selenite (IV) and selenate (VI) in the water, the differences in bioaccumulation, effects (e.g., oxidative stress, antioxidants etc.) and molecular mechanisms between Se(IV) and Se(VI) have been relatively understudied in wild fish. In this study, the P. parva were exposed to waterborne Se (10, 200 and 1000 μg/L of Se(IV) or Se(VI)) and sampled at 4, 14 and 28 days. Bioaccumulation, tissue distributions of Se and following effects in different tissues were evaluated. The results showed that the levels of Se in the gills and intestine were significantly elevated with a seemingly concentration-dependent pattern in the Se(IV) treatment, with respectively 173.3% and 57.2% increase after 28 days of exposure, relative to that of Se(VI) treatment. Additionally, significant accumulation of Se was also observed in the muscle of Se(IV) treated fish. Se exposure increased the MDA levels in the brain and gills in the Se(IV) treatment, but less apparent in the Se(VI) treatment. Meanwhile, Se exposure lowered (at least 56%) the activity of GST in the gills, but increased the activity of AChE in the muscle (~69%) and brain (~50%) after 28 d. Most importantly, after 28 d of exposure, Se exposure caused significant decrease in GSH levels in the gills (at least 35%) and in all tissues examined at the highest test concentration. In general, the results showed that Se(IV) led to faster accumulation of Se than Se(VI) in P. parva, and the resulted lipid peroxidation was closely related to the levels of antioxidants, especially GSH. Our results suggest that the ecotoxicological effects of waterborne selenite and selenate differ in this freshwater species in the field.
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spelling pubmed-61386502018-09-15 The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva Ma, Shanshan Zeng, Xiangfeng Chen, Hongxing Geng, Shicong Yan, Liang Luo, Yongju Xie, Lingtian Zhang, Qianru Sci Rep Article Selenium (Se) might be protective against oxidative stress at nutritional levels, but elevated Se concentrations in the diet has been revealed as the main culprit for the extinction of natural fish populations in Se-contaminated lakes. Though Se predominate as waterborne selenite (IV) and selenate (VI) in the water, the differences in bioaccumulation, effects (e.g., oxidative stress, antioxidants etc.) and molecular mechanisms between Se(IV) and Se(VI) have been relatively understudied in wild fish. In this study, the P. parva were exposed to waterborne Se (10, 200 and 1000 μg/L of Se(IV) or Se(VI)) and sampled at 4, 14 and 28 days. Bioaccumulation, tissue distributions of Se and following effects in different tissues were evaluated. The results showed that the levels of Se in the gills and intestine were significantly elevated with a seemingly concentration-dependent pattern in the Se(IV) treatment, with respectively 173.3% and 57.2% increase after 28 days of exposure, relative to that of Se(VI) treatment. Additionally, significant accumulation of Se was also observed in the muscle of Se(IV) treated fish. Se exposure increased the MDA levels in the brain and gills in the Se(IV) treatment, but less apparent in the Se(VI) treatment. Meanwhile, Se exposure lowered (at least 56%) the activity of GST in the gills, but increased the activity of AChE in the muscle (~69%) and brain (~50%) after 28 d. Most importantly, after 28 d of exposure, Se exposure caused significant decrease in GSH levels in the gills (at least 35%) and in all tissues examined at the highest test concentration. In general, the results showed that Se(IV) led to faster accumulation of Se than Se(VI) in P. parva, and the resulted lipid peroxidation was closely related to the levels of antioxidants, especially GSH. Our results suggest that the ecotoxicological effects of waterborne selenite and selenate differ in this freshwater species in the field. Nature Publishing Group UK 2018-09-14 /pmc/articles/PMC6138650/ /pubmed/30218092 http://dx.doi.org/10.1038/s41598-018-32270-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ma, Shanshan
Zeng, Xiangfeng
Chen, Hongxing
Geng, Shicong
Yan, Liang
Luo, Yongju
Xie, Lingtian
Zhang, Qianru
The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva
title The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva
title_full The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva
title_fullStr The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva
title_full_unstemmed The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva
title_short The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva
title_sort differences in bioaccumulation and effects between se(iv) and se(vi) in the topmouth gudgeon pseudorasbora parva
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138650/
https://www.ncbi.nlm.nih.gov/pubmed/30218092
http://dx.doi.org/10.1038/s41598-018-32270-z
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