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The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome

Chemerin is an adipocyte derived signalling molecule (adipokine) that serves as a ligand activator of Chemokine-like receptor 1(CMKLR1). Chemerin/CMKLR1 signalling is well established to regulate fundamental processes in metabolism and inflammation. The composition and function of gut microbiota has...

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Autores principales: Dranse, Helen J., Zheng, Ashlee, Comeau, André M., Langille, Morgan G.I., Zabel, Brian A., Sinal, Christopher J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139019/
https://www.ncbi.nlm.nih.gov/pubmed/30225164
http://dx.doi.org/10.7717/peerj.5494
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author Dranse, Helen J.
Zheng, Ashlee
Comeau, André M.
Langille, Morgan G.I.
Zabel, Brian A.
Sinal, Christopher J.
author_facet Dranse, Helen J.
Zheng, Ashlee
Comeau, André M.
Langille, Morgan G.I.
Zabel, Brian A.
Sinal, Christopher J.
author_sort Dranse, Helen J.
collection PubMed
description Chemerin is an adipocyte derived signalling molecule (adipokine) that serves as a ligand activator of Chemokine-like receptor 1(CMKLR1). Chemerin/CMKLR1 signalling is well established to regulate fundamental processes in metabolism and inflammation. The composition and function of gut microbiota has also been shown to impact the development of metabolic and inflammatory diseases such as obesity, diabetes and inflammatory bowel disease. In this study, we assessed the microbiome composition of fecal samples isolated from wildtype, chemerin, or CMKLR1 knockout mice using Illumina-based sequencing. Moreover, the knockout mice and respective wildtype mice used in this study were housed at different universities allowing us to compare facility-dependent effects on microbiome composition. While there was no difference in alpha diversity within samples when compared by either facility or genotype, we observed a dramatic difference in the presence and abundance of numerous taxa between facilities. There were minor differences in bacterial abundance between wildtype and chemerin knockout mice, but significantly more differences in taxa abundance between wildtype and CMKLR1 knockout mice. Specifically, CMKLR1 knockout mice exhibited decreased abundance of Akkermansia and Prevotella, which correlated with body weight in CMKLR1 knockout, but not wildtype mice. This is the first study to investigate a linkage between chemerin/CMKLR1 signaling and microbiome composition. The results of our study suggest that chemerin/CMKLR1 signaling influences metabolic processes through effects on the gut microbiome. Furthermore, the dramatic difference in microbiome composition between facilities might contribute to discrepancies in the metabolic phenotype of CMKLR1 knockout mice reported by independent groups. Considered altogether, these findings establish a foundation for future studies to investigate the relationship between chemerin signaling and the gut microbiome on the development and progression of metabolic and inflammatory disease.
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spelling pubmed-61390192018-09-17 The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome Dranse, Helen J. Zheng, Ashlee Comeau, André M. Langille, Morgan G.I. Zabel, Brian A. Sinal, Christopher J. PeerJ Bioinformatics Chemerin is an adipocyte derived signalling molecule (adipokine) that serves as a ligand activator of Chemokine-like receptor 1(CMKLR1). Chemerin/CMKLR1 signalling is well established to regulate fundamental processes in metabolism and inflammation. The composition and function of gut microbiota has also been shown to impact the development of metabolic and inflammatory diseases such as obesity, diabetes and inflammatory bowel disease. In this study, we assessed the microbiome composition of fecal samples isolated from wildtype, chemerin, or CMKLR1 knockout mice using Illumina-based sequencing. Moreover, the knockout mice and respective wildtype mice used in this study were housed at different universities allowing us to compare facility-dependent effects on microbiome composition. While there was no difference in alpha diversity within samples when compared by either facility or genotype, we observed a dramatic difference in the presence and abundance of numerous taxa between facilities. There were minor differences in bacterial abundance between wildtype and chemerin knockout mice, but significantly more differences in taxa abundance between wildtype and CMKLR1 knockout mice. Specifically, CMKLR1 knockout mice exhibited decreased abundance of Akkermansia and Prevotella, which correlated with body weight in CMKLR1 knockout, but not wildtype mice. This is the first study to investigate a linkage between chemerin/CMKLR1 signaling and microbiome composition. The results of our study suggest that chemerin/CMKLR1 signaling influences metabolic processes through effects on the gut microbiome. Furthermore, the dramatic difference in microbiome composition between facilities might contribute to discrepancies in the metabolic phenotype of CMKLR1 knockout mice reported by independent groups. Considered altogether, these findings establish a foundation for future studies to investigate the relationship between chemerin signaling and the gut microbiome on the development and progression of metabolic and inflammatory disease. PeerJ Inc. 2018-09-12 /pmc/articles/PMC6139019/ /pubmed/30225164 http://dx.doi.org/10.7717/peerj.5494 Text en ©2018 Dranse et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Dranse, Helen J.
Zheng, Ashlee
Comeau, André M.
Langille, Morgan G.I.
Zabel, Brian A.
Sinal, Christopher J.
The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
title The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
title_full The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
title_fullStr The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
title_full_unstemmed The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
title_short The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
title_sort impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139019/
https://www.ncbi.nlm.nih.gov/pubmed/30225164
http://dx.doi.org/10.7717/peerj.5494
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