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Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon

As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper...

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Autores principales: Liu, Qingxin, Fan, Jialong, Zhou, Chuang, Wang, Liqun, Zhao, Bin, Zhang, Haibin, Liu, Bin, Tong, Chunyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139239/
https://www.ncbi.nlm.nih.gov/pubmed/30245721
http://dx.doi.org/10.1155/2018/3625823
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author Liu, Qingxin
Fan, Jialong
Zhou, Chuang
Wang, Liqun
Zhao, Bin
Zhang, Haibin
Liu, Bin
Tong, Chunyi
author_facet Liu, Qingxin
Fan, Jialong
Zhou, Chuang
Wang, Liqun
Zhao, Bin
Zhang, Haibin
Liu, Bin
Tong, Chunyi
author_sort Liu, Qingxin
collection PubMed
description As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA.
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spelling pubmed-61392392018-09-23 Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon Liu, Qingxin Fan, Jialong Zhou, Chuang Wang, Liqun Zhao, Bin Zhang, Haibin Liu, Bin Tong, Chunyi Int J Anal Chem Research Article As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA. Hindawi 2018-09-02 /pmc/articles/PMC6139239/ /pubmed/30245721 http://dx.doi.org/10.1155/2018/3625823 Text en Copyright © 2018 Qingxin Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Qingxin
Fan, Jialong
Zhou, Chuang
Wang, Liqun
Zhao, Bin
Zhang, Haibin
Liu, Bin
Tong, Chunyi
Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
title Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
title_full Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
title_fullStr Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
title_full_unstemmed Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
title_short Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
title_sort quantitative detection of mirna-21 expression in tumor cells and tissues based on molecular beacon
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139239/
https://www.ncbi.nlm.nih.gov/pubmed/30245721
http://dx.doi.org/10.1155/2018/3625823
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