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Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139239/ https://www.ncbi.nlm.nih.gov/pubmed/30245721 http://dx.doi.org/10.1155/2018/3625823 |
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author | Liu, Qingxin Fan, Jialong Zhou, Chuang Wang, Liqun Zhao, Bin Zhang, Haibin Liu, Bin Tong, Chunyi |
author_facet | Liu, Qingxin Fan, Jialong Zhou, Chuang Wang, Liqun Zhao, Bin Zhang, Haibin Liu, Bin Tong, Chunyi |
author_sort | Liu, Qingxin |
collection | PubMed |
description | As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA. |
format | Online Article Text |
id | pubmed-6139239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-61392392018-09-23 Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon Liu, Qingxin Fan, Jialong Zhou, Chuang Wang, Liqun Zhao, Bin Zhang, Haibin Liu, Bin Tong, Chunyi Int J Anal Chem Research Article As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA. Hindawi 2018-09-02 /pmc/articles/PMC6139239/ /pubmed/30245721 http://dx.doi.org/10.1155/2018/3625823 Text en Copyright © 2018 Qingxin Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Qingxin Fan, Jialong Zhou, Chuang Wang, Liqun Zhao, Bin Zhang, Haibin Liu, Bin Tong, Chunyi Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
title | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
title_full | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
title_fullStr | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
title_full_unstemmed | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
title_short | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
title_sort | quantitative detection of mirna-21 expression in tumor cells and tissues based on molecular beacon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139239/ https://www.ncbi.nlm.nih.gov/pubmed/30245721 http://dx.doi.org/10.1155/2018/3625823 |
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