Cargando…
Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea
Microalgae are considered as sustainable resources for biofuel production. However, recently the focus on microalgal research has shifted toward the investigation of high-value metabolites for potential pharmaceutical and nutritional applications. Herein, we report the identification of a novel olea...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139321/ https://www.ncbi.nlm.nih.gov/pubmed/30250461 http://dx.doi.org/10.3389/fmicb.2018.02159 |
_version_ | 1783355493654200320 |
---|---|
author | Lee, Seungki Lim, Se Ra Jeong, Dae Gwin Kim, Ji Hyung |
author_facet | Lee, Seungki Lim, Se Ra Jeong, Dae Gwin Kim, Ji Hyung |
author_sort | Lee, Seungki |
collection | PubMed |
description | Microalgae are considered as sustainable resources for biofuel production. However, recently the focus on microalgal research has shifted toward the investigation of high-value metabolites for potential pharmaceutical and nutritional applications. Herein, we report the identification of a novel oleaginous green microalga isolated from the Yellow Sea in Korea. We also describe the morphological, molecular, and biochemical characteristics of this microalga. On the basis of microscopic and genetic analyses, the isolate was classified as Lobosphaera incisa (the strain was designated as K-1), and molecular phylogeny revealed that the isolate distinctly differed from the other known L. incisa strains. The microalga could be cultivated in various commercial culture media under a relatively broad range of pH and temperature conditions. We also did a rough and detailed estimation of the different cellular components in the microalga. The composition of arachidonic acid (C20:4ω6) in the lipids of L. incisa strain K-1 was relatively high, similar to that in other strains, however, the K-1 strain had higher proportions of the ω3 series of fatty acids (FAs), including α-linolenic acid (C18:3ω3) and eicosapentaenoic acid (C20:5ω3), highlighting its uniqueness and strong potential for biotechnological application. To the best of our knowledge, this is the first report on the isolation of L. incisa from Korea as well as from a marine environment; this novel strain might be useful for the production of high-value ω3 and ω6 polyunsaturated fatty acids (PUFAs). |
format | Online Article Text |
id | pubmed-6139321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-61393212018-09-24 Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea Lee, Seungki Lim, Se Ra Jeong, Dae Gwin Kim, Ji Hyung Front Microbiol Microbiology Microalgae are considered as sustainable resources for biofuel production. However, recently the focus on microalgal research has shifted toward the investigation of high-value metabolites for potential pharmaceutical and nutritional applications. Herein, we report the identification of a novel oleaginous green microalga isolated from the Yellow Sea in Korea. We also describe the morphological, molecular, and biochemical characteristics of this microalga. On the basis of microscopic and genetic analyses, the isolate was classified as Lobosphaera incisa (the strain was designated as K-1), and molecular phylogeny revealed that the isolate distinctly differed from the other known L. incisa strains. The microalga could be cultivated in various commercial culture media under a relatively broad range of pH and temperature conditions. We also did a rough and detailed estimation of the different cellular components in the microalga. The composition of arachidonic acid (C20:4ω6) in the lipids of L. incisa strain K-1 was relatively high, similar to that in other strains, however, the K-1 strain had higher proportions of the ω3 series of fatty acids (FAs), including α-linolenic acid (C18:3ω3) and eicosapentaenoic acid (C20:5ω3), highlighting its uniqueness and strong potential for biotechnological application. To the best of our knowledge, this is the first report on the isolation of L. incisa from Korea as well as from a marine environment; this novel strain might be useful for the production of high-value ω3 and ω6 polyunsaturated fatty acids (PUFAs). Frontiers Media S.A. 2018-09-10 /pmc/articles/PMC6139321/ /pubmed/30250461 http://dx.doi.org/10.3389/fmicb.2018.02159 Text en Copyright © 2018 Lee, Lim, Jeong and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lee, Seungki Lim, Se Ra Jeong, Dae Gwin Kim, Ji Hyung Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea |
title | Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea |
title_full | Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea |
title_fullStr | Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea |
title_full_unstemmed | Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea |
title_short | Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea |
title_sort | characterization of an oleaginous unicellular green microalga, lobosphaera incisa (reisigl, 1964) strain k-1, isolated from a tidal flat in the yellow sea, republic of korea |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139321/ https://www.ncbi.nlm.nih.gov/pubmed/30250461 http://dx.doi.org/10.3389/fmicb.2018.02159 |
work_keys_str_mv | AT leeseungki characterizationofanoleaginousunicellulargreenmicroalgalobosphaeraincisareisigl1964straink1isolatedfromatidalflatintheyellowsearepublicofkorea AT limsera characterizationofanoleaginousunicellulargreenmicroalgalobosphaeraincisareisigl1964straink1isolatedfromatidalflatintheyellowsearepublicofkorea AT jeongdaegwin characterizationofanoleaginousunicellulargreenmicroalgalobosphaeraincisareisigl1964straink1isolatedfromatidalflatintheyellowsearepublicofkorea AT kimjihyung characterizationofanoleaginousunicellulargreenmicroalgalobosphaeraincisareisigl1964straink1isolatedfromatidalflatintheyellowsearepublicofkorea |