Cargando…
Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr)
The Arabian leopard (Panthera pardus nimr) is critically endangered by the International Union for Conservation of Nature, with an effective population of 150-250 across its entire range in the Arabian Peninsula. Isolation and preservation of multipotent mesenchymal stem cells is beneficial both for...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140384/ https://www.ncbi.nlm.nih.gov/pubmed/30237980 http://dx.doi.org/10.4314/ovj.v8i3.12 |
_version_ | 1783355581824761856 |
---|---|
author | Golachowski, Andrzej Hashmi, Sulaiman Al Golachowska, Barbara |
author_facet | Golachowski, Andrzej Hashmi, Sulaiman Al Golachowska, Barbara |
author_sort | Golachowski, Andrzej |
collection | PubMed |
description | The Arabian leopard (Panthera pardus nimr) is critically endangered by the International Union for Conservation of Nature, with an effective population of 150-250 across its entire range in the Arabian Peninsula. Isolation and preservation of multipotent mesenchymal stem cells is beneficial both for medical and research purposes. The optimal protocol for collection, handling, culturing and preserving the Arabian leopard mesenchymal stem cells acquired from bone marrow was established. Anesthesia with combination of medetomidine and tiletamine-zozalepam is the safest option even for old animals with concurrent disease including chronic kidney disease. |
format | Online Article Text |
id | pubmed-6140384 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-61403842018-09-20 Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) Golachowski, Andrzej Hashmi, Sulaiman Al Golachowska, Barbara Open Vet J Short Communication The Arabian leopard (Panthera pardus nimr) is critically endangered by the International Union for Conservation of Nature, with an effective population of 150-250 across its entire range in the Arabian Peninsula. Isolation and preservation of multipotent mesenchymal stem cells is beneficial both for medical and research purposes. The optimal protocol for collection, handling, culturing and preserving the Arabian leopard mesenchymal stem cells acquired from bone marrow was established. Anesthesia with combination of medetomidine and tiletamine-zozalepam is the safest option even for old animals with concurrent disease including chronic kidney disease. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2018 2018-09-10 /pmc/articles/PMC6140384/ /pubmed/30237980 http://dx.doi.org/10.4314/ovj.v8i3.12 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License. |
spellingShingle | Short Communication Golachowski, Andrzej Hashmi, Sulaiman Al Golachowska, Barbara Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) |
title | Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) |
title_full | Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) |
title_fullStr | Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) |
title_full_unstemmed | Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) |
title_short | Isolation and preservation of multipotent mesenchymal stem cells from bone marrow of Arabian leopard (Panthera pradus nimr) |
title_sort | isolation and preservation of multipotent mesenchymal stem cells from bone marrow of arabian leopard (panthera pradus nimr) |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140384/ https://www.ncbi.nlm.nih.gov/pubmed/30237980 http://dx.doi.org/10.4314/ovj.v8i3.12 |
work_keys_str_mv | AT golachowskiandrzej isolationandpreservationofmultipotentmesenchymalstemcellsfrombonemarrowofarabianleopardpantherapradusnimr AT hashmisulaimanal isolationandpreservationofmultipotentmesenchymalstemcellsfrombonemarrowofarabianleopardpantherapradusnimr AT golachowskabarbara isolationandpreservationofmultipotentmesenchymalstemcellsfrombonemarrowofarabianleopardpantherapradusnimr |