Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose

The dataset presented in this article is related to the research article entitled “One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose” (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigat...

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Autores principales: Bawn, Maria, Subrizi, Fabiana, Lye, Gary J., Sheppard, Tom D., Hailes, Helen C., Ward, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Pharmacology, Toxicology and Pharmaceutical Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140825/
https://www.ncbi.nlm.nih.gov/pubmed/30229010
http://dx.doi.org/10.1016/j.dib.2018.05.140
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author Bawn, Maria
Subrizi, Fabiana
Lye, Gary J.
Sheppard, Tom D.
Hailes, Helen C.
Ward, John M.
author_facet Bawn, Maria
Subrizi, Fabiana
Lye, Gary J.
Sheppard, Tom D.
Hailes, Helen C.
Ward, John M.
author_sort Bawn, Maria
collection PubMed
description The dataset presented in this article is related to the research article entitled “One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose” (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to characterized TAms with desired activity. DNA and amino acid sequences are presented for all the enzymes investigated. Calibration curves for products of the TK and TAm reactions are also presented along with chromatographic analysis of the various one-pot reactions.
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spelling pubmed-61408252018-09-18 Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose Bawn, Maria Subrizi, Fabiana Lye, Gary J. Sheppard, Tom D. Hailes, Helen C. Ward, John M. Data Brief Pharmacology, Toxicology and Pharmaceutical Science The dataset presented in this article is related to the research article entitled “One-pot, two-step transaminase and transketolase synthesis of l-gluco-heptulose from l-arabinose” (Bawn et al., 2018 in press) [1]. This article presents data on initial experiments that were carried out to investigate new thermostable transketolase (TK) activities with l-arabinose. Transaminase (TAm) sequences from an in-house library of thermophilic strains were analyzed to compare homologies to characterized TAms with desired activity. DNA and amino acid sequences are presented for all the enzymes investigated. Calibration curves for products of the TK and TAm reactions are also presented along with chromatographic analysis of the various one-pot reactions. Elsevier 2018-05-31 /pmc/articles/PMC6140825/ /pubmed/30229010 http://dx.doi.org/10.1016/j.dib.2018.05.140 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Pharmacology, Toxicology and Pharmaceutical Science
Bawn, Maria
Subrizi, Fabiana
Lye, Gary J.
Sheppard, Tom D.
Hailes, Helen C.
Ward, John M.
Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
title Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
title_full Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
title_fullStr Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
title_full_unstemmed Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
title_short Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
title_sort data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
topic Pharmacology, Toxicology and Pharmaceutical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6140825/
https://www.ncbi.nlm.nih.gov/pubmed/30229010
http://dx.doi.org/10.1016/j.dib.2018.05.140
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