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A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND ME...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141113/ https://www.ncbi.nlm.nih.gov/pubmed/30254460 http://dx.doi.org/10.2147/OTT.S160531 |
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author | Ying Zhi, Lu Xu, Zhang Ning, Li Jia Jin, Li Hai Cui, Yan Hong, Huang Guan Fang, Xin Jian |
author_facet | Ying Zhi, Lu Xu, Zhang Ning, Li Jia Jin, Li Hai Cui, Yan Hong, Huang Guan Fang, Xin Jian |
author_sort | Ying Zhi, Lu |
collection | PubMed |
description | BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND METHODS: MCF-7 cells transfected with the eukaryotic expression vector pcDNA3.1-CD44st (MCF/CD44st) were used to examine the effect of the activation of the HA-CD44st-transforming growth factor β (TGFβ)-phosphatidylinositol-3-kinase (PI3K) signaling pathway on the invasive ability of MCF-7 cells. The expression of proteins related to this signaling pathway was assessed by flow cytometry, reverse transcription-polymerase chain reaction, and Western blotting, and the role of AP-1 in the pathway was investigated by electrophoretic mobility shift assay. The effect of pathway activation on the invasion of MCF-7 cells was assessed by Transwell assay, and CD44 expression in breast cancer tissue was detected by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of CD44st and HER-2 in breast cancer tissue and their correlation was investigated. RESULTS: HA significantly upregulated HER-2 and TGFβ in MCF-7/CD44st cells, increased p-AKT expression and AP-1 activity, and promoted the invasive ability of tumor cells. CD44st mRNA expression had significant difference between breast cancer tissues and adjacent normal tissues (P < 0.05), and high expression of CD44st mRNA was closely correlated with HER-2 expression in breast cancer tissues. CONCLUSION: Binding of HA to the CD44st receptor may regulate the invasiveness of MCF-7 cells through the CD44st/TGFβ/PI3K/AP-1 signaling pathway with increased expression of TGFβ and HER-2. The expression of CD44st mRNA is correlated with HER-2 expression in postoperative breast cancer patients. |
format | Online Article Text |
id | pubmed-6141113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61411132018-09-25 A correlation study of the expression of HA-CD44st and HER-2 in breast cancer Ying Zhi, Lu Xu, Zhang Ning, Li Jia Jin, Li Hai Cui, Yan Hong, Huang Guan Fang, Xin Jian Onco Targets Ther Original Research BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND METHODS: MCF-7 cells transfected with the eukaryotic expression vector pcDNA3.1-CD44st (MCF/CD44st) were used to examine the effect of the activation of the HA-CD44st-transforming growth factor β (TGFβ)-phosphatidylinositol-3-kinase (PI3K) signaling pathway on the invasive ability of MCF-7 cells. The expression of proteins related to this signaling pathway was assessed by flow cytometry, reverse transcription-polymerase chain reaction, and Western blotting, and the role of AP-1 in the pathway was investigated by electrophoretic mobility shift assay. The effect of pathway activation on the invasion of MCF-7 cells was assessed by Transwell assay, and CD44 expression in breast cancer tissue was detected by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of CD44st and HER-2 in breast cancer tissue and their correlation was investigated. RESULTS: HA significantly upregulated HER-2 and TGFβ in MCF-7/CD44st cells, increased p-AKT expression and AP-1 activity, and promoted the invasive ability of tumor cells. CD44st mRNA expression had significant difference between breast cancer tissues and adjacent normal tissues (P < 0.05), and high expression of CD44st mRNA was closely correlated with HER-2 expression in breast cancer tissues. CONCLUSION: Binding of HA to the CD44st receptor may regulate the invasiveness of MCF-7 cells through the CD44st/TGFβ/PI3K/AP-1 signaling pathway with increased expression of TGFβ and HER-2. The expression of CD44st mRNA is correlated with HER-2 expression in postoperative breast cancer patients. Dove Medical Press 2018-09-10 /pmc/articles/PMC6141113/ /pubmed/30254460 http://dx.doi.org/10.2147/OTT.S160531 Text en © 2018 Zhi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Ying Zhi, Lu Xu, Zhang Ning, Li Jia Jin, Li Hai Cui, Yan Hong, Huang Guan Fang, Xin Jian A correlation study of the expression of HA-CD44st and HER-2 in breast cancer |
title | A correlation study of the expression of HA-CD44st and HER-2 in breast cancer |
title_full | A correlation study of the expression of HA-CD44st and HER-2 in breast cancer |
title_fullStr | A correlation study of the expression of HA-CD44st and HER-2 in breast cancer |
title_full_unstemmed | A correlation study of the expression of HA-CD44st and HER-2 in breast cancer |
title_short | A correlation study of the expression of HA-CD44st and HER-2 in breast cancer |
title_sort | correlation study of the expression of ha-cd44st and her-2 in breast cancer |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141113/ https://www.ncbi.nlm.nih.gov/pubmed/30254460 http://dx.doi.org/10.2147/OTT.S160531 |
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