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A correlation study of the expression of HA-CD44st and HER-2 in breast cancer

BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND ME...

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Autores principales: Ying Zhi, Lu, Xu, Zhang, Ning, Li, Jia Jin, Li, Hai Cui, Yan, Hong, Huang Guan, Fang, Xin Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141113/
https://www.ncbi.nlm.nih.gov/pubmed/30254460
http://dx.doi.org/10.2147/OTT.S160531
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author Ying Zhi, Lu
Xu, Zhang
Ning, Li
Jia Jin, Li
Hai Cui, Yan
Hong, Huang Guan
Fang, Xin Jian
author_facet Ying Zhi, Lu
Xu, Zhang
Ning, Li
Jia Jin, Li
Hai Cui, Yan
Hong, Huang Guan
Fang, Xin Jian
author_sort Ying Zhi, Lu
collection PubMed
description BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND METHODS: MCF-7 cells transfected with the eukaryotic expression vector pcDNA3.1-CD44st (MCF/CD44st) were used to examine the effect of the activation of the HA-CD44st-transforming growth factor β (TGFβ)-phosphatidylinositol-3-kinase (PI3K) signaling pathway on the invasive ability of MCF-7 cells. The expression of proteins related to this signaling pathway was assessed by flow cytometry, reverse transcription-polymerase chain reaction, and Western blotting, and the role of AP-1 in the pathway was investigated by electrophoretic mobility shift assay. The effect of pathway activation on the invasion of MCF-7 cells was assessed by Transwell assay, and CD44 expression in breast cancer tissue was detected by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of CD44st and HER-2 in breast cancer tissue and their correlation was investigated. RESULTS: HA significantly upregulated HER-2 and TGFβ in MCF-7/CD44st cells, increased p-AKT expression and AP-1 activity, and promoted the invasive ability of tumor cells. CD44st mRNA expression had significant difference between breast cancer tissues and adjacent normal tissues (P < 0.05), and high expression of CD44st mRNA was closely correlated with HER-2 expression in breast cancer tissues. CONCLUSION: Binding of HA to the CD44st receptor may regulate the invasiveness of MCF-7 cells through the CD44st/TGFβ/PI3K/AP-1 signaling pathway with increased expression of TGFβ and HER-2. The expression of CD44st mRNA is correlated with HER-2 expression in postoperative breast cancer patients.
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spelling pubmed-61411132018-09-25 A correlation study of the expression of HA-CD44st and HER-2 in breast cancer Ying Zhi, Lu Xu, Zhang Ning, Li Jia Jin, Li Hai Cui, Yan Hong, Huang Guan Fang, Xin Jian Onco Targets Ther Original Research BACKGROUND: This study investigated the effect of hyaluronic acid (HA)-CD44st on the invasive ability of human breast cancer MCF-7 cells and the correlation between the expression of CD44st and human epidermal growth factor receptor-2 (HER-2) in postoperative breast cancer patients. MATERIALS AND METHODS: MCF-7 cells transfected with the eukaryotic expression vector pcDNA3.1-CD44st (MCF/CD44st) were used to examine the effect of the activation of the HA-CD44st-transforming growth factor β (TGFβ)-phosphatidylinositol-3-kinase (PI3K) signaling pathway on the invasive ability of MCF-7 cells. The expression of proteins related to this signaling pathway was assessed by flow cytometry, reverse transcription-polymerase chain reaction, and Western blotting, and the role of AP-1 in the pathway was investigated by electrophoretic mobility shift assay. The effect of pathway activation on the invasion of MCF-7 cells was assessed by Transwell assay, and CD44 expression in breast cancer tissue was detected by immunohistochemistry. Quantitative reverse transcription-polymerase chain reaction was used to detect the expression of CD44st and HER-2 in breast cancer tissue and their correlation was investigated. RESULTS: HA significantly upregulated HER-2 and TGFβ in MCF-7/CD44st cells, increased p-AKT expression and AP-1 activity, and promoted the invasive ability of tumor cells. CD44st mRNA expression had significant difference between breast cancer tissues and adjacent normal tissues (P < 0.05), and high expression of CD44st mRNA was closely correlated with HER-2 expression in breast cancer tissues. CONCLUSION: Binding of HA to the CD44st receptor may regulate the invasiveness of MCF-7 cells through the CD44st/TGFβ/PI3K/AP-1 signaling pathway with increased expression of TGFβ and HER-2. The expression of CD44st mRNA is correlated with HER-2 expression in postoperative breast cancer patients. Dove Medical Press 2018-09-10 /pmc/articles/PMC6141113/ /pubmed/30254460 http://dx.doi.org/10.2147/OTT.S160531 Text en © 2018 Zhi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Ying Zhi, Lu
Xu, Zhang
Ning, Li
Jia Jin, Li
Hai Cui, Yan
Hong, Huang Guan
Fang, Xin Jian
A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
title A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
title_full A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
title_fullStr A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
title_full_unstemmed A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
title_short A correlation study of the expression of HA-CD44st and HER-2 in breast cancer
title_sort correlation study of the expression of ha-cd44st and her-2 in breast cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141113/
https://www.ncbi.nlm.nih.gov/pubmed/30254460
http://dx.doi.org/10.2147/OTT.S160531
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