HPLC-ESI-MS method for C(60) fullerene mitochondrial content quantification

The presented dataset describes the quantification of carbon nanoparticle C(60) fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed...

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Detalles Bibliográficos
Autores principales: Grebinyk, Anna, Grebinyk, Sergii, Prylutska, Svitlana, Ritter, Uwe, Matyshevska, Olga, Dandekar, Thomas, Frohme, Marcus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Medicine and Dentistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141383/
https://www.ncbi.nlm.nih.gov/pubmed/30229080
http://dx.doi.org/10.1016/j.dib.2018.06.089
Descripción
Sumario:The presented dataset describes the quantification of carbon nanoparticle C(60) fullerene accumulated in mitochondria of human leukemic cells treated with nanostructure. Firstly, the high performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC-ESI-MS) method was developed for quantitative analysis of pristine C(60) fullerene. Then, human leukemic cells were incubated with C(60) fullerene, homogenized and subjected to the differential centrifugation to retrieve mitochondrial fraction. The C(60) fullerene content was quantified by HPLC-ESI-MS in extracts of cellular fractions. This data article refers to the research article “C(60) Fullerene Accumulation in Human Leukemic Cells and Perspectives of LED-mediated Photodynamic Therapy” by Grebinyk et al. [1].

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