Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging

The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rh...

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Autores principales: Xu, Kaiyu, Chen, Dong, Wang, Zhengbo, Ma, Jian, Zhou, Jian, Chen, Nanhui, Lv, Longbao, Zheng, Yongtang, Hu, Xintian, Zhang, Yi, Li, Jiali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141548/
https://www.ncbi.nlm.nih.gov/pubmed/30245844
http://dx.doi.org/10.1038/s41421-018-0050-1
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author Xu, Kaiyu
Chen, Dong
Wang, Zhengbo
Ma, Jian
Zhou, Jian
Chen, Nanhui
Lv, Longbao
Zheng, Yongtang
Hu, Xintian
Zhang, Yi
Li, Jiali
author_facet Xu, Kaiyu
Chen, Dong
Wang, Zhengbo
Ma, Jian
Zhou, Jian
Chen, Nanhui
Lv, Longbao
Zheng, Yongtang
Hu, Xintian
Zhang, Yi
Li, Jiali
author_sort Xu, Kaiyu
collection PubMed
description The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rhesus macaque (macaca mulatta) brain in two age groups from adult (10 y) to aged (20 y) periods. Total 17,050 well expressed, stable circRNAs were identified. Cluster analysis reveals that dynamic changes in circRNA expression show the spatial-, sex- and age-biased specificities. On the basis of separate profiling of the RNAs, age-related circRNAs show differential correlation to host mRNA expression. Furthermore, two voltage-dependent L- and R-type calcium channel gene-derived circCACNA2D1 and circCACNA1E negatively regulate their host mRNA expression. Our results demonstrate the power of changes in circRNA expression to reveal insights into a potentially circRNA-mediated regulatory mechanism underlying the biology of brain aging.
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spelling pubmed-61415482018-09-21 Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging Xu, Kaiyu Chen, Dong Wang, Zhengbo Ma, Jian Zhou, Jian Chen, Nanhui Lv, Longbao Zheng, Yongtang Hu, Xintian Zhang, Yi Li, Jiali Cell Discov Article The abundance and function of circular RNAs (circRNAs) in mammalian brain have been reported, but their alterations in the biology of brain aging remain elusive. Here, using deep RNA profiling with linear RNA digestion by RNase R we explored a comprehensive map of changes in circRNA expression in rhesus macaque (macaca mulatta) brain in two age groups from adult (10 y) to aged (20 y) periods. Total 17,050 well expressed, stable circRNAs were identified. Cluster analysis reveals that dynamic changes in circRNA expression show the spatial-, sex- and age-biased specificities. On the basis of separate profiling of the RNAs, age-related circRNAs show differential correlation to host mRNA expression. Furthermore, two voltage-dependent L- and R-type calcium channel gene-derived circCACNA2D1 and circCACNA1E negatively regulate their host mRNA expression. Our results demonstrate the power of changes in circRNA expression to reveal insights into a potentially circRNA-mediated regulatory mechanism underlying the biology of brain aging. Nature Publishing Group UK 2018-09-18 /pmc/articles/PMC6141548/ /pubmed/30245844 http://dx.doi.org/10.1038/s41421-018-0050-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xu, Kaiyu
Chen, Dong
Wang, Zhengbo
Ma, Jian
Zhou, Jian
Chen, Nanhui
Lv, Longbao
Zheng, Yongtang
Hu, Xintian
Zhang, Yi
Li, Jiali
Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging
title Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging
title_full Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging
title_fullStr Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging
title_full_unstemmed Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging
title_short Annotation and functional clustering of circRNA expression in rhesus macaque brain during aging
title_sort annotation and functional clustering of circrna expression in rhesus macaque brain during aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141548/
https://www.ncbi.nlm.nih.gov/pubmed/30245844
http://dx.doi.org/10.1038/s41421-018-0050-1
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