Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications

In this study, we report on the fabrication of multilayered tri-functional magnetic-SERS-fluorescence nanoprobes (MF-SERS particles) containing clustered superparamagnetic Fe(3)O(4) nanoparticles (NPs), silver NPs, and a fluorescent silica layer. The MF-SERS particles exhibited strong SERS signals f...

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Autores principales: Kim, Hyung-Mo, Kim, Dong-Min, Jeong, Cheolhwan, Park, So Yeon, Cha, Myeong Geun, Ha, Yuna, Jang, Dahye, Kyeong, San, Pham, Xuan-Hung, Hahm, Eunil, Lee, Sang Hun, Jeong, Dae Hong, Lee, Yoon-Sik, Kim, Dong-Eun, Jun, Bong-Hyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141549/
https://www.ncbi.nlm.nih.gov/pubmed/30224683
http://dx.doi.org/10.1038/s41598-018-32044-7
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author Kim, Hyung-Mo
Kim, Dong-Min
Jeong, Cheolhwan
Park, So Yeon
Cha, Myeong Geun
Ha, Yuna
Jang, Dahye
Kyeong, San
Pham, Xuan-Hung
Hahm, Eunil
Lee, Sang Hun
Jeong, Dae Hong
Lee, Yoon-Sik
Kim, Dong-Eun
Jun, Bong-Hyun
author_facet Kim, Hyung-Mo
Kim, Dong-Min
Jeong, Cheolhwan
Park, So Yeon
Cha, Myeong Geun
Ha, Yuna
Jang, Dahye
Kyeong, San
Pham, Xuan-Hung
Hahm, Eunil
Lee, Sang Hun
Jeong, Dae Hong
Lee, Yoon-Sik
Kim, Dong-Eun
Jun, Bong-Hyun
author_sort Kim, Hyung-Mo
collection PubMed
description In this study, we report on the fabrication of multilayered tri-functional magnetic-SERS-fluorescence nanoprobes (MF-SERS particles) containing clustered superparamagnetic Fe(3)O(4) nanoparticles (NPs), silver NPs, and a fluorescent silica layer. The MF-SERS particles exhibited strong SERS signals from the silver NPs as well as both superparamagnetism and fluorescence. MF–SERS particles were uptaken by cells, allowing successful separation using an external magnetic field. SERS and fluorescence signals could be detected from the NP-containing cells, and CD44 antibody-conjugated MF-SERS particles selectively targeted MDA-MB-231 cells. Based on these properties, MF-SERS particles proved to be a useful nanoprobe for multiplex detection and separation of cancer cells.
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spelling pubmed-61415492018-09-20 Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications Kim, Hyung-Mo Kim, Dong-Min Jeong, Cheolhwan Park, So Yeon Cha, Myeong Geun Ha, Yuna Jang, Dahye Kyeong, San Pham, Xuan-Hung Hahm, Eunil Lee, Sang Hun Jeong, Dae Hong Lee, Yoon-Sik Kim, Dong-Eun Jun, Bong-Hyun Sci Rep Article In this study, we report on the fabrication of multilayered tri-functional magnetic-SERS-fluorescence nanoprobes (MF-SERS particles) containing clustered superparamagnetic Fe(3)O(4) nanoparticles (NPs), silver NPs, and a fluorescent silica layer. The MF-SERS particles exhibited strong SERS signals from the silver NPs as well as both superparamagnetism and fluorescence. MF–SERS particles were uptaken by cells, allowing successful separation using an external magnetic field. SERS and fluorescence signals could be detected from the NP-containing cells, and CD44 antibody-conjugated MF-SERS particles selectively targeted MDA-MB-231 cells. Based on these properties, MF-SERS particles proved to be a useful nanoprobe for multiplex detection and separation of cancer cells. Nature Publishing Group UK 2018-09-17 /pmc/articles/PMC6141549/ /pubmed/30224683 http://dx.doi.org/10.1038/s41598-018-32044-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kim, Hyung-Mo
Kim, Dong-Min
Jeong, Cheolhwan
Park, So Yeon
Cha, Myeong Geun
Ha, Yuna
Jang, Dahye
Kyeong, San
Pham, Xuan-Hung
Hahm, Eunil
Lee, Sang Hun
Jeong, Dae Hong
Lee, Yoon-Sik
Kim, Dong-Eun
Jun, Bong-Hyun
Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
title Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
title_full Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
title_fullStr Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
title_full_unstemmed Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
title_short Assembly of Plasmonic and Magnetic Nanoparticles with Fluorescent Silica Shell Layer for Tri-functional SERS-Magnetic-Fluorescence Probes and Its Bioapplications
title_sort assembly of plasmonic and magnetic nanoparticles with fluorescent silica shell layer for tri-functional sers-magnetic-fluorescence probes and its bioapplications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141549/
https://www.ncbi.nlm.nih.gov/pubmed/30224683
http://dx.doi.org/10.1038/s41598-018-32044-7
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