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Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

Light-sheet fluorescence microscopy (LSFM) has emerged as a powerful method for rapid and optically efficient 3D microscopy. Initial LSFM designs utilized a static sheet of light, termed selective plane illumination microscopy (SPIM), which exhibited shadowing artifacts and deteriorated contrast due...

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Detalles Bibliográficos
Autores principales: Glaser, Adam K., Chen, Ye, Yin, Chengbo, Wei, Linpeng, Barner, Lindsey A., Reder, Nicholas P., Liu, Jonathan T. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141597/
https://www.ncbi.nlm.nih.gov/pubmed/30224740
http://dx.doi.org/10.1038/s41598-018-32367-5