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Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS

A very sensitive LC-MS/MS assay was developed implementing a liquid-liquid extraction step followed by mass spectrometry which was operated in both positive and negative ion modes. The assay was calibrated with readily available commercial calibrators and compared with international reference standa...

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Detalles Bibliográficos
Autores principales: Schofield, Ryan C., Ramanathan, Lakshmi V., Pessin, Melissa S., Carlow, Dean C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141700/
https://www.ncbi.nlm.nih.gov/pubmed/30246107
http://dx.doi.org/10.1016/j.dib.2018.08.077
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author Schofield, Ryan C.
Ramanathan, Lakshmi V.
Pessin, Melissa S.
Carlow, Dean C.
author_facet Schofield, Ryan C.
Ramanathan, Lakshmi V.
Pessin, Melissa S.
Carlow, Dean C.
author_sort Schofield, Ryan C.
collection PubMed
description A very sensitive LC-MS/MS assay was developed implementing a liquid-liquid extraction step followed by mass spectrometry which was operated in both positive and negative ion modes. The assay was calibrated with readily available commercial calibrators and compared with international reference standards. This data is also presented in “Sensitive Simultaneous Quantitation of Testosterone and Estradiol in Serum by LC-MS/MS without Derivatization and Comparison with the CDC HoSt Program” (Schofield et al., 2017). This article includes the comparison of the LC-MS/MS assay with a commonly available chemiluminescencent immunoassay for the quantitation of both estradiol and testosterone. In addition we show baseline separation of estradiol and testosterone from other structurally related and/or isobaric compounds that could potentially interfere with the assay. In addition, various calibrator materials were tested and compared with internationally-recognized reference materials.
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spelling pubmed-61417002018-09-21 Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS Schofield, Ryan C. Ramanathan, Lakshmi V. Pessin, Melissa S. Carlow, Dean C. Data Brief Chemistry A very sensitive LC-MS/MS assay was developed implementing a liquid-liquid extraction step followed by mass spectrometry which was operated in both positive and negative ion modes. The assay was calibrated with readily available commercial calibrators and compared with international reference standards. This data is also presented in “Sensitive Simultaneous Quantitation of Testosterone and Estradiol in Serum by LC-MS/MS without Derivatization and Comparison with the CDC HoSt Program” (Schofield et al., 2017). This article includes the comparison of the LC-MS/MS assay with a commonly available chemiluminescencent immunoassay for the quantitation of both estradiol and testosterone. In addition we show baseline separation of estradiol and testosterone from other structurally related and/or isobaric compounds that could potentially interfere with the assay. In addition, various calibrator materials were tested and compared with internationally-recognized reference materials. Elsevier 2018-08-30 /pmc/articles/PMC6141700/ /pubmed/30246107 http://dx.doi.org/10.1016/j.dib.2018.08.077 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Chemistry
Schofield, Ryan C.
Ramanathan, Lakshmi V.
Pessin, Melissa S.
Carlow, Dean C.
Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
title Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
title_full Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
title_fullStr Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
title_full_unstemmed Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
title_short Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS
title_sort data to support a simultaneous testosterone and estradiol assay in serum by lc-ms/ms
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141700/
https://www.ncbi.nlm.nih.gov/pubmed/30246107
http://dx.doi.org/10.1016/j.dib.2018.08.077
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