Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments

The data presented here corresponds to the research paper “Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague using environmental DNA”. A crayfish-specific assay was designed and optimised using three real-time PCR supermixes (SYBR™ Green, Ss...

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Autores principales: Robinson, Chloe Victoria, Uren Webster, Tamsyn M., Consuegra, Sofia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Environmental Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141759/
https://www.ncbi.nlm.nih.gov/pubmed/30246065
http://dx.doi.org/10.1016/j.dib.2018.05.134
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author Robinson, Chloe Victoria
Uren Webster, Tamsyn M.
Consuegra, Sofia
author_facet Robinson, Chloe Victoria
Uren Webster, Tamsyn M.
Consuegra, Sofia
author_sort Robinson, Chloe Victoria
collection PubMed
description The data presented here corresponds to the research paper “Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague using environmental DNA”. A crayfish-specific assay was designed and optimised using three real-time PCR supermixes (SYBR™ Green, SsoFast™ EvaGreen® and HOT FIREPol® EvaGreen®). Diagnostic high resolution melt (HRM) data from direct application of assay on both ex-situ eDNA water samples and field samples from four catchments (two in Wales, two in England) is presented in this article, displaying positive HRM profiles for invasive signal crayfish (Pacifastacus leniusculus), native white-clawed crayfish (Austropotamobius pallipes) and crayfish plague causal agent (Aphanomyces astaci).
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spelling pubmed-61417592018-09-21 Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments Robinson, Chloe Victoria Uren Webster, Tamsyn M. Consuegra, Sofia Data Brief Environmental Science The data presented here corresponds to the research paper “Simultaneous detection of invasive signal crayfish, endangered white-clawed crayfish and the crayfish plague using environmental DNA”. A crayfish-specific assay was designed and optimised using three real-time PCR supermixes (SYBR™ Green, SsoFast™ EvaGreen® and HOT FIREPol® EvaGreen®). Diagnostic high resolution melt (HRM) data from direct application of assay on both ex-situ eDNA water samples and field samples from four catchments (two in Wales, two in England) is presented in this article, displaying positive HRM profiles for invasive signal crayfish (Pacifastacus leniusculus), native white-clawed crayfish (Austropotamobius pallipes) and crayfish plague causal agent (Aphanomyces astaci). Elsevier 2018-05-29 /pmc/articles/PMC6141759/ /pubmed/30246065 http://dx.doi.org/10.1016/j.dib.2018.05.134 Text en © 2018 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Environmental Science
Robinson, Chloe Victoria
Uren Webster, Tamsyn M.
Consuegra, Sofia
Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
title Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
title_full Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
title_fullStr Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
title_full_unstemmed Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
title_short Data on optimisation of a multiplex HRM-qPCR assay for native and invasive crayfish as well as the crayfish plague in four river catchments
title_sort data on optimisation of a multiplex hrm-qpcr assay for native and invasive crayfish as well as the crayfish plague in four river catchments
topic Environmental Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141759/
https://www.ncbi.nlm.nih.gov/pubmed/30246065
http://dx.doi.org/10.1016/j.dib.2018.05.134
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