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Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense

Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticill...

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Autores principales: Li, Ting-Gang, Zhang, Dan-Dan, Zhou, Lei, Kong, Zhi-Qiang, Hussaini, Adamu S., Wang, Dan, Li, Jun-Jiao, Short, Dylan P. G., Dhar, Nikhilesh, Klosterman, Steven J., Wang, Bao-Li, Yin, Chun-Mei, Subbarao, Krishna V., Chen, Jie-Yin, Dai, Xiao-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141769/
https://www.ncbi.nlm.nih.gov/pubmed/30254650
http://dx.doi.org/10.3389/fpls.2018.01266
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author Li, Ting-Gang
Zhang, Dan-Dan
Zhou, Lei
Kong, Zhi-Qiang
Hussaini, Adamu S.
Wang, Dan
Li, Jun-Jiao
Short, Dylan P. G.
Dhar, Nikhilesh
Klosterman, Steven J.
Wang, Bao-Li
Yin, Chun-Mei
Subbarao, Krishna V.
Chen, Jie-Yin
Dai, Xiao-Feng
author_facet Li, Ting-Gang
Zhang, Dan-Dan
Zhou, Lei
Kong, Zhi-Qiang
Hussaini, Adamu S.
Wang, Dan
Li, Jun-Jiao
Short, Dylan P. G.
Dhar, Nikhilesh
Klosterman, Steven J.
Wang, Bao-Li
Yin, Chun-Mei
Subbarao, Krishna V.
Chen, Jie-Yin
Dai, Xiao-Feng
author_sort Li, Ting-Gang
collection PubMed
description Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense.
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spelling pubmed-61417692018-09-25 Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense Li, Ting-Gang Zhang, Dan-Dan Zhou, Lei Kong, Zhi-Qiang Hussaini, Adamu S. Wang, Dan Li, Jun-Jiao Short, Dylan P. G. Dhar, Nikhilesh Klosterman, Steven J. Wang, Bao-Li Yin, Chun-Mei Subbarao, Krishna V. Chen, Jie-Yin Dai, Xiao-Feng Front Plant Sci Plant Science Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense. Frontiers Media S.A. 2018-09-11 /pmc/articles/PMC6141769/ /pubmed/30254650 http://dx.doi.org/10.3389/fpls.2018.01266 Text en Copyright © 2018 Li, Zhang, Zhou, Kong, Hussaini, Wang, Li, Short, Dhar, Klosterman, Wang, Yin, Subbarao, Chen and Dai. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Li, Ting-Gang
Zhang, Dan-Dan
Zhou, Lei
Kong, Zhi-Qiang
Hussaini, Adamu S.
Wang, Dan
Li, Jun-Jiao
Short, Dylan P. G.
Dhar, Nikhilesh
Klosterman, Steven J.
Wang, Bao-Li
Yin, Chun-Mei
Subbarao, Krishna V.
Chen, Jie-Yin
Dai, Xiao-Feng
Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
title Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
title_full Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
title_fullStr Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
title_full_unstemmed Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
title_short Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
title_sort genome-wide identification and functional analyses of the crk gene family in cotton reveals gbcrk18 confers verticillium wilt resistance in gossypium barbadense
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6141769/
https://www.ncbi.nlm.nih.gov/pubmed/30254650
http://dx.doi.org/10.3389/fpls.2018.01266
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