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Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam

To examine transmission dynamics of Mtb isolated from TB patients in Ho Chi Minh City, Vietnam we sequenced whole genomes of 1,635 isolates and compared these with 3,144 isolates from elsewhere. The data reveal an underlying burden of disease caused by endemic Mtb Lineage 1 associated with activatio...

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Autores principales: Holt, Kathryn E, McAdam, Paul, Thai, Phan Vuong Khac, Thuong, Nguyen Thuy Thuong, Ha, Dang Thi Minh, Lan, Nguyen Ngoc, Lan, Nguyen Huu, Nhu, Nguyen Thi Quynh, Hai, Hoang Thanh, Ha, Vu Thi Ngoc, Thwaites, Guy, Edwards, David J, Nath, Artika P, Pham, Kym, Ascher, David B, Farrar, Jeremy, Khor, Chiea Chuen, Teo, Yik Ying, Inouye, Michael, Caws, Maxine, Dunstan, Sarah J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143168/
https://www.ncbi.nlm.nih.gov/pubmed/29785015
http://dx.doi.org/10.1038/s41588-018-0117-9
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author Holt, Kathryn E
McAdam, Paul
Thai, Phan Vuong Khac
Thuong, Nguyen Thuy Thuong
Ha, Dang Thi Minh
Lan, Nguyen Ngoc
Lan, Nguyen Huu
Nhu, Nguyen Thi Quynh
Hai, Hoang Thanh
Ha, Vu Thi Ngoc
Thwaites, Guy
Edwards, David J
Nath, Artika P
Pham, Kym
Ascher, David B
Farrar, Jeremy
Khor, Chiea Chuen
Teo, Yik Ying
Inouye, Michael
Caws, Maxine
Dunstan, Sarah J
author_facet Holt, Kathryn E
McAdam, Paul
Thai, Phan Vuong Khac
Thuong, Nguyen Thuy Thuong
Ha, Dang Thi Minh
Lan, Nguyen Ngoc
Lan, Nguyen Huu
Nhu, Nguyen Thi Quynh
Hai, Hoang Thanh
Ha, Vu Thi Ngoc
Thwaites, Guy
Edwards, David J
Nath, Artika P
Pham, Kym
Ascher, David B
Farrar, Jeremy
Khor, Chiea Chuen
Teo, Yik Ying
Inouye, Michael
Caws, Maxine
Dunstan, Sarah J
author_sort Holt, Kathryn E
collection PubMed
description To examine transmission dynamics of Mtb isolated from TB patients in Ho Chi Minh City, Vietnam we sequenced whole genomes of 1,635 isolates and compared these with 3,144 isolates from elsewhere. The data reveal an underlying burden of disease caused by endemic Mtb Lineage 1 associated with activation of long-term latent infection, and a three-fold higher burden associated with more recently introduced Beijing lineage and Lineage 4 Mtb strains. We find that Beijing lineage Mtb is frequently transferred between Vietnam and other countries, and detect higher levels of transmission of Beijing lineage strains within this host population than endemic Lineage 1 Mtb. Screening for parallel evolution of Beijing lineage-associated SNPs in other Mtb lineages as a signal of positive selection, we identify a mutation in the ESX-5 type VII secreted protein EsxW, which could potentially contribute to the enhanced transmission of Beijing lineage Mtb in Vietnamese and other host populations.
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spelling pubmed-61431682018-11-21 Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam Holt, Kathryn E McAdam, Paul Thai, Phan Vuong Khac Thuong, Nguyen Thuy Thuong Ha, Dang Thi Minh Lan, Nguyen Ngoc Lan, Nguyen Huu Nhu, Nguyen Thi Quynh Hai, Hoang Thanh Ha, Vu Thi Ngoc Thwaites, Guy Edwards, David J Nath, Artika P Pham, Kym Ascher, David B Farrar, Jeremy Khor, Chiea Chuen Teo, Yik Ying Inouye, Michael Caws, Maxine Dunstan, Sarah J Nat Genet Article To examine transmission dynamics of Mtb isolated from TB patients in Ho Chi Minh City, Vietnam we sequenced whole genomes of 1,635 isolates and compared these with 3,144 isolates from elsewhere. The data reveal an underlying burden of disease caused by endemic Mtb Lineage 1 associated with activation of long-term latent infection, and a three-fold higher burden associated with more recently introduced Beijing lineage and Lineage 4 Mtb strains. We find that Beijing lineage Mtb is frequently transferred between Vietnam and other countries, and detect higher levels of transmission of Beijing lineage strains within this host population than endemic Lineage 1 Mtb. Screening for parallel evolution of Beijing lineage-associated SNPs in other Mtb lineages as a signal of positive selection, we identify a mutation in the ESX-5 type VII secreted protein EsxW, which could potentially contribute to the enhanced transmission of Beijing lineage Mtb in Vietnamese and other host populations. 2018-05-21 2018-06 /pmc/articles/PMC6143168/ /pubmed/29785015 http://dx.doi.org/10.1038/s41588-018-0117-9 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Holt, Kathryn E
McAdam, Paul
Thai, Phan Vuong Khac
Thuong, Nguyen Thuy Thuong
Ha, Dang Thi Minh
Lan, Nguyen Ngoc
Lan, Nguyen Huu
Nhu, Nguyen Thi Quynh
Hai, Hoang Thanh
Ha, Vu Thi Ngoc
Thwaites, Guy
Edwards, David J
Nath, Artika P
Pham, Kym
Ascher, David B
Farrar, Jeremy
Khor, Chiea Chuen
Teo, Yik Ying
Inouye, Michael
Caws, Maxine
Dunstan, Sarah J
Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam
title Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam
title_full Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam
title_fullStr Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam
title_full_unstemmed Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam
title_short Frequent transmission of the Mycobacterium tuberculosis Beijing lineage and positive selection for EsxW Beijing variant in Vietnam
title_sort frequent transmission of the mycobacterium tuberculosis beijing lineage and positive selection for esxw beijing variant in vietnam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143168/
https://www.ncbi.nlm.nih.gov/pubmed/29785015
http://dx.doi.org/10.1038/s41588-018-0117-9
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