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Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model
Dental caries is caused by acids released from bacterial biofilms. However, the in vivo formation of initial biofilms in relation to caries remains largely unexplored. The aim of this study was to compare the oral microbiome during the initial phase of bacterial colonization for individuals with (CC...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143549/ https://www.ncbi.nlm.nih.gov/pubmed/30228377 http://dx.doi.org/10.1038/s41598-018-32361-x |
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author | Rupf, Stefan Laczny, Cedric C. Galata, Valentina Backes, Christina Keller, Andreas Umanskaya, Natalia Erol, Arzu Tierling, Sascha Lo Porto, Christina Walter, Jörn Kirsch, Jasmin Hannig, Matthias Hannig, Christian |
author_facet | Rupf, Stefan Laczny, Cedric C. Galata, Valentina Backes, Christina Keller, Andreas Umanskaya, Natalia Erol, Arzu Tierling, Sascha Lo Porto, Christina Walter, Jörn Kirsch, Jasmin Hannig, Matthias Hannig, Christian |
author_sort | Rupf, Stefan |
collection | PubMed |
description | Dental caries is caused by acids released from bacterial biofilms. However, the in vivo formation of initial biofilms in relation to caries remains largely unexplored. The aim of this study was to compare the oral microbiome during the initial phase of bacterial colonization for individuals with (CC) and without (NC) cavitated dentin caries lesions. Bovine enamel slabs on acrylic splints were worn by the volunteers (CC: 14, NC: 13) for in situ biofilm formation (2 h, 4 h, 8 h, 1 ml saliva as reference). Sequencing of the V1/V2 regions of the 16S rRNA gene was performed (MiSeq). The relative abundances of individual operational taxonomic units (OTUs) were compared between samples from the CC group and the NC group. Random forests models were furthermore trained to separate the groups. While the overall heterogeneity did not differ substantially between CC and NC individuals, several individual OTUs were found to have significantly different relative abundances. For the 8 h samples, most of the significant OTUs showed higher relative abundances in the CC group, while the majority of significant OTUs in the saliva samples were more abundant in the NC group. Furthermore, using OTU signatures enabled a separation between both groups, with area-under-the-curve (AUC) values of ~0.8. In summary, the results suggest that initial oral biofilms provide the potential to differentiate between CC and NC individuals. |
format | Online Article Text |
id | pubmed-6143549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-61435492018-09-24 Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model Rupf, Stefan Laczny, Cedric C. Galata, Valentina Backes, Christina Keller, Andreas Umanskaya, Natalia Erol, Arzu Tierling, Sascha Lo Porto, Christina Walter, Jörn Kirsch, Jasmin Hannig, Matthias Hannig, Christian Sci Rep Article Dental caries is caused by acids released from bacterial biofilms. However, the in vivo formation of initial biofilms in relation to caries remains largely unexplored. The aim of this study was to compare the oral microbiome during the initial phase of bacterial colonization for individuals with (CC) and without (NC) cavitated dentin caries lesions. Bovine enamel slabs on acrylic splints were worn by the volunteers (CC: 14, NC: 13) for in situ biofilm formation (2 h, 4 h, 8 h, 1 ml saliva as reference). Sequencing of the V1/V2 regions of the 16S rRNA gene was performed (MiSeq). The relative abundances of individual operational taxonomic units (OTUs) were compared between samples from the CC group and the NC group. Random forests models were furthermore trained to separate the groups. While the overall heterogeneity did not differ substantially between CC and NC individuals, several individual OTUs were found to have significantly different relative abundances. For the 8 h samples, most of the significant OTUs showed higher relative abundances in the CC group, while the majority of significant OTUs in the saliva samples were more abundant in the NC group. Furthermore, using OTU signatures enabled a separation between both groups, with area-under-the-curve (AUC) values of ~0.8. In summary, the results suggest that initial oral biofilms provide the potential to differentiate between CC and NC individuals. Nature Publishing Group UK 2018-09-18 /pmc/articles/PMC6143549/ /pubmed/30228377 http://dx.doi.org/10.1038/s41598-018-32361-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Rupf, Stefan Laczny, Cedric C. Galata, Valentina Backes, Christina Keller, Andreas Umanskaya, Natalia Erol, Arzu Tierling, Sascha Lo Porto, Christina Walter, Jörn Kirsch, Jasmin Hannig, Matthias Hannig, Christian Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
title | Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
title_full | Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
title_fullStr | Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
title_full_unstemmed | Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
title_short | Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
title_sort | comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6143549/ https://www.ncbi.nlm.nih.gov/pubmed/30228377 http://dx.doi.org/10.1038/s41598-018-32361-x |
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